Literature DB >> 20732882

A novel method for large-scale immuno-SEM using protein G coupled polystyrene beads.

In-Hwan Song1, James E Dennis.   

Abstract

Polystyrene beads with a mean diameter of 0.76 μm were coupled with protein G and then anti-type II collagen IgG or anti-chondroitin-4-sulphate IgG were tagged to protein G. Antibody-tagged beads were applied to articular cartilage and labelled beads were counted in each sample. Antibody-tagged beads labelled significantly higher than IgG isotype control. We propose immuno-SEM using protein G coupled beads as a valuable method for micrometre range observation for specific protein distribution on surfaces of tissues or organs. This will provide information about structure as well as antigenicity on the surface at the same time.

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Year:  2010        PMID： 20732882      PMCID： PMC3156677          DOI： 10.1093/jmicro/dfq064

Source DB:  PubMed          Journal:  J Electron Microsc (Tokyo)        ISSN： 0022-0744


  7 in total

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Authors:  James E Dennis; Nir Cohen; Victor M Goldberg; Arnold I Caplan
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1.  Correlating Fluorescence and High-Resolution Scanning Electron Microscopy (HRSEM) for the study of GABAA receptor clustering induced by inhibitory synaptic plasticity.

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Journal:  Sci Rep       Date:  2017-10-23       Impact factor: 4.379
  1 in total

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