BACKGROUND: One of the most studied actions of juvenile hormone (JH) is its ability to modulate ecdysteroid signaling during insect development and metamorphosis. Previous studies in mosquitoes showed that 20-hydroxyecdysone (20E) regulates vitellogenin synthesis. However, the action of JH and its mimics, e.g. methoprene, on female reproduction of mosquitoes remains unknown. RESULTS: Here, a major malaria vector, Anopheles gambiae Giles, was used as a model insect to study the action of methoprene on female reproduction. Ecdysteroid titers and expression profiles of ecdysone-regulated genes were determined before and after a blood meal. An ecdysteroid peak was detected at 12 h post blood meal (PBM). The maximum expression of ecdysone-regulated genes, such as ecdysone receptor (EcR), hormone receptor 3 (HR3) and vitellogenin (Vg) gene, coincided with the ecdysteroid peak. Interestingly, topical application of methoprene at 6 h PBM delayed ovarian development and egg maturation by suppressing the expression of ecdysone-regulated genes in female mosquitoes. CONCLUSION: The data suggest that ecdysteroid titers are correlated with Vg synthesis, and methoprene affects vitellogenesis by modulating ecdysteroid action in A. gambiae. (c) 2010 Society of Chemical Industry.
BACKGROUND: One of the most studied actions of juvenile hormone (JH) is its ability to modulate ecdysteroid signaling during insect development and metamorphosis. Previous studies in mosquitoes showed that 20-hydroxyecdysone (20E) regulates vitellogenin synthesis. However, the action of JH and its mimics, e.g. methoprene, on female reproduction of mosquitoes remains unknown. RESULTS: Here, a major malaria vector, Anopheles gambiae Giles, was used as a model insect to study the action of methoprene on female reproduction. Ecdysteroid titers and expression profiles of ecdysone-regulated genes were determined before and after a blood meal. An ecdysteroid peak was detected at 12 h post blood meal (PBM). The maximum expression of ecdysone-regulated genes, such as ecdysone receptor (EcR), hormone receptor 3 (HR3) and vitellogenin (Vg) gene, coincided with the ecdysteroid peak. Interestingly, topical application of methoprene at 6 h PBM delayed ovarian development and egg maturation by suppressing the expression of ecdysone-regulated genes in female mosquitoes. CONCLUSION: The data suggest that ecdysteroid titers are correlated with Vg synthesis, and methoprene affects vitellogenesis by modulating ecdysteroid action in A. gambiae. (c) 2010 Society of Chemical Industry.
Authors: Kristine Werling; W Robert Shaw; Maurice A Itoe; Kathleen A Westervelt; Perrine Marcenac; Douglas G Paton; Duo Peng; Naresh Singh; Andrea L Smidler; Adam South; Amy A Deik; Liliana Mancio-Silva; Allison R Demas; Sandra March; Eric Calvo; Sangeeta N Bhatia; Clary B Clish; Flaminia Catteruccia Journal: Cell Date: 2019-03-28 Impact factor: 41.582
Authors: Joseph P Receveur; Jennifer L Pechal; M Eric Benbow; Gary Donato; Tadhgh Rainey; John R Wallace Journal: Microb Ecol Date: 2018-03-17 Impact factor: 4.552
Authors: W Robert Shaw; Eleonora Teodori; Sara N Mitchell; Francesco Baldini; Paolo Gabrieli; David W Rogers; Flaminia Catteruccia Journal: Proc Natl Acad Sci U S A Date: 2014-04-07 Impact factor: 11.205
Authors: Sara N Mitchell; Evdoxia G Kakani; Adam South; Paul I Howell; Robert M Waterhouse; Flaminia Catteruccia Journal: Science Date: 2015-02-27 Impact factor: 47.728