David W Johnson1. 1. SA Pathology, Department of Genetic Medicine, Women's and Children's Hospital, 72 King William Rd, North Adelaide, South Australia 5006, Australia. david.johnson@adelaide.edu.au
Abstract
OBJECTIVES: To develop a tandem mass spectrometry (MS/MS) method for plasma free and total carnitine, incorporating acid hydrolysis for total carnitine measurement, for improved accuracy and sample throughput. DESIGN AND METHODS: Limit of detection (LOD), limit of quantitation (LOQ), linearity, accuracy, precision coefficient of variation (CV), method comparison with a radioenzymatic assay (REA) and liquid chromatography tandem mass spectrometry assay (LC-MS/MS) were performed. The method was tested for interferences and for long-term performance. RESULTS: The method LOD and LOQ were 0.87 and 1.36 μmol/L for free carnitine and 1.79 and 2.54 μmol/L for total carnitine, respectively. No interferences were detected. Long-term measurement CVs for both free and total carnitine were <4%. CONCLUSIONS: The method shows acceptable performance characteristics with improved speed and low level accuracy compared to existing REA and MS/MS methods.
OBJECTIVES: To develop a tandem mass spectrometry (MS/MS) method for plasma free and total carnitine, incorporating acid hydrolysis for total carnitine measurement, for improved accuracy and sample throughput. DESIGN AND METHODS: Limit of detection (LOD), limit of quantitation (LOQ), linearity, accuracy, precision coefficient of variation (CV), method comparison with a radioenzymatic assay (REA) and liquid chromatography tandem mass spectrometry assay (LC-MS/MS) were performed. The method was tested for interferences and for long-term performance. RESULTS: The method LOD and LOQ were 0.87 and 1.36 μmol/L for free carnitine and 1.79 and 2.54 μmol/L for total carnitine, respectively. No interferences were detected. Long-term measurement CVs for both free and total carnitine were <4%. CONCLUSIONS: The method shows acceptable performance characteristics with improved speed and low level accuracy compared to existing REA and MS/MS methods.
Authors: Gloria Schlegel; Janine Keller; Frank Hirche; Stefanie Geissler; Frieder J Schwarz; Robert Ringseis; Gabriele I Stangl; Klaus Eder Journal: BMC Vet Res Date: 2012-03-14 Impact factor: 2.741