Literature DB >> 20720026

Critical stages of extracting DNA from Aspergillus fumigatus in whole-blood specimens.

P Lewis White1, Michael D Perry, Juergen Loeffler, Willem Melchers, Lena Klingspor, Stephane Bretagne, Elaine McCulloch, Manuel Cuenca-Estrella, Niklas Finnstrom, J Peter Donnelly, Rosemary A Barnes.   

Abstract

A standardized protocol for extracting DNA from Aspergillus fumigatus has been proposed by the European Aspergillus PCR Initiative (EAPCRI). Using meta-regression analysis, the EAPCRI showed certain stages of the process to be critical to providing a satisfactory analytical sensitivity. The study investigated each step of the EAPCRI protocol by elimination and monitored the influence on Aspergillus PCR performance.

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Year:  2010        PMID： 20720026      PMCID： PMC2953093          DOI： 10.1128/JCM.01466-10

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  6 in total

1.  Quantification of fungal DNA by using fluorescence resonance energy transfer and the light cycler system.

Authors:  J Loeffler; N Henke; H Hebart; D Schmidt; L Hagmeyer; U Schumacher; H Einsele
Journal:  J Clin Microbiol       Date:  2000-02       Impact factor: 5.948

2.  Use of real-time PCR to process the first galactomannan-positive serum sample in diagnosing invasive aspergillosis.

Authors:  Laurence Millon; Renaud Piarroux; Eric Deconinck; Claude-Eric Bulabois; Frédéric Grenouillet; Pierre Rohrlich; Jean-Marc Costa; Stéphane Bretagne
Journal:  J Clin Microbiol       Date:  2005-10       Impact factor: 5.948

3.  Aspergillus PCR: one step closer to standardization.

Authors:  P Lewis White; Stéphane Bretagne; Lena Klingspor; Willem J G Melchers; Elaine McCulloch; Bettina Schulz; Niklas Finnstrom; Carlo Mengoli; Rosemary A Barnes; J Peter Donnelly; Juergen Loeffler
Journal:  J Clin Microbiol       Date:  2010-02-10       Impact factor: 5.948

4.  The evolution and evaluation of a whole blood polymerase chain reaction assay for the detection of invasive aspergillosis in hematology patients in a routine clinical setting.

Authors:  P Lewis White; Christopher J Linton; Michael D Perry; Elizabeth M Johnson; Rosemary A Barnes
Journal:  Clin Infect Dis       Date:  2006-01-17       Impact factor: 9.079

5.  Strain-dependent variation in 18S ribosomal DNA Copy numbers in Aspergillus fumigatus.

Authors:  M L Herrera; A C Vallor; J A Gelfond; T F Patterson; B L Wickes
Journal:  J Clin Microbiol       Date:  2009-03-04       Impact factor: 5.948

6.  Revised definitions of invasive fungal disease from the European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) Consensus Group.

Authors:  Ben De Pauw; Thomas J Walsh; J Peter Donnelly; David A Stevens; John E Edwards; Thierry Calandra; Peter G Pappas; Johan Maertens; Olivier Lortholary; Carol A Kauffman; David W Denning; Thomas F Patterson; Georg Maschmeyer; Jacques Bille; William E Dismukes; Raoul Herbrecht; William W Hope; Christopher C Kibbler; Bart Jan Kullberg; Kieren A Marr; Patricia Muñoz; Frank C Odds; John R Perfect; Angela Restrepo; Markus Ruhnke; Brahm H Segal; Jack D Sobel; Tania C Sorrell; Claudio Viscoli; John R Wingard; Theoklis Zaoutis; John E Bennett
Journal:  Clin Infect Dis       Date:  2008-06-15       Impact factor: 9.079
  6 in total
  24 in total

1.  A novel extraction method combining plasma with a whole-blood fraction shows excellent sensitivity and reproducibility for patients at high risk for invasive aspergillosis.

Authors:  Jan Springer; Hannes Schloßnagel; Werner Heinz; Thomas Doedt; Rainer Soeller; Hermann Einsele; Juergen Loeffler
Journal:  J Clin Microbiol       Date:  2012-05-16       Impact factor: 5.948

2.  Clinical utility and development of biomarkers in invasive aspergillosis.

Authors:  Thomas F Patterson
Journal:  Trans Am Clin Climatol Assoc       Date:  2011

3.  Clinical performance of a commercial real-time PCR assay for Aspergillus DNA detection in serum samples from high-risk patients: comparison with a galactomannan enzyme immunoassay.

Authors:  P Pini; C Bettua; C F Orsi; C Venturelli; L Faglioni; F Forghieri; S Bigliardi; F Luppi; M Girardis; E Blasi
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2014-08-01       Impact factor: 3.267

4.  Not over yet: fungal infections following methyl prednisolone injections smoulder on.

Authors:  Rosemary Barnes; P Lewis White
Journal:  J Clin Microbiol       Date:  2014-09       Impact factor: 5.948

Review 5.  Molecular and nonmolecular diagnostic methods for invasive fungal infections.

Authors:  Marios Arvanitis; Theodora Anagnostou; Beth Burgwyn Fuchs; Angela M Caliendo; Eleftherios Mylonakis
Journal:  Clin Microbiol Rev       Date:  2014-07       Impact factor: 26.132

Review 6.  Molecular Diagnostic Testing for Aspergillus.

Authors:  Margaret V Powers-Fletcher; Kimberly E Hanson
Journal:  J Clin Microbiol       Date:  2016-08-03       Impact factor: 5.948

7.  Has Aspergillus PCR Come to the Age of Maturity?

Authors:  Alessandro C Pasqualotto; Diego R Falci
Journal:  Mycopathologia       Date:  2016-06-29       Impact factor: 2.574

8.  Evaluation of analytical and preliminary clinical performance of Myconostica MycAssay Aspergillus when testing serum specimens for diagnosis of invasive Aspergillosis.

Authors:  P Lewis White; Michael D Perry; Adrian Moody; Sarah A Follett; Gillian Morgan; Rosemary A Barnes
Journal:  J Clin Microbiol       Date:  2011-03-30       Impact factor: 5.948

9.  Detection of fungal DNA in human body fluids and tissues during a multistate outbreak of fungal meningitis and other infections.

Authors:  Lalitha Gade; Christina M Scheel; Cau D Pham; Mark D Lindsley; Naureen Iqbal; Angela Ahlquist Cleveland; Anne M Whitney; Shawn R Lockhart; Mary E Brandt; Anastasia P Litvintseva
Journal:  Eukaryot Cell       Date:  2013-03-01

10.  Multicenter comparison of serum and whole-blood specimens for detection of Aspergillus DNA in high-risk hematological patients.

Authors:  Jan Springer; C O Morton; Michael Perry; Werner J Heinz; Melinda Paholcsek; Mona Alzheimer; T R Rogers; Rosemary A Barnes; Hermann Einsele; Juergen Loeffler; P Lewis White
Journal:  J Clin Microbiol       Date:  2013-02-20       Impact factor: 5.948
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