OBJECTIVES: To compare the cuff technique to traditional suture technique in establishing cervical heart transplant model in mice. MATERIALS AND METHODS: Eighty transplants were performed by 1 surgeon, 40 using the cuff technique, and 40 using the suture technique, under the same circumstances. RESULTS: The cuff approach was significantly superior to conventional suture anastomosis in higher surgical successful rate, less surgery, and less ischemic time (P < .05). Suture anastomosis required an intensive microsurgical training and at least a 16x surgical microscope, while the cuff anastomosis required less learning time and a 10x surgical microscope. CONCLUSIONS: The cuff technique is the preferred method in cervical heart transplant model in mice.
OBJECTIVES: To compare the cuff technique to traditional suture technique in establishing cervical heart transplant model in mice. MATERIALS AND METHODS: Eighty transplants were performed by 1 surgeon, 40 using the cuff technique, and 40 using the suture technique, under the same circumstances. RESULTS: The cuff approach was significantly superior to conventional suture anastomosis in higher surgical successful rate, less surgery, and less ischemic time (P < .05). Suture anastomosis required an intensive microsurgical training and at least a 16x surgical microscope, while the cuff anastomosis required less learning time and a 10x surgical microscope. CONCLUSIONS: The cuff technique is the preferred method in cervical heart transplant model in mice.
Authors: Benno Cardini; Rupert Oberhuber; Sven R Hein; Rebecca Eiter; Martin Hermann; Markus Kofler; Stefan Schneeberger; Gerald Brandacher; Manuel Maglione Journal: J Vis Exp Date: 2017-12-16 Impact factor: 1.355
Authors: Rupert Oberhuber; Benno Cardini; Markus Kofler; Paul Ritschl; Robert Oellinger; Felix Aigner; Robert Sucher; Stefan Schneeberger; Johann Pratschke; Gerald Brandacher; Manuel Maglione Journal: J Vis Exp Date: 2014-10-12 Impact factor: 1.355