Literature DB >> 2071158

Yeast artificial chromosome vectors for efficient clone manipulation and mapping.

J H Shero1, M K McCormick, S E Antonarakis, P Hieter.   

Abstract

The yeast artificial chromosome (YAC) cloning system allows the cloning of exogenous DNA several hundred kilobases in length. To enhance the usefulness of this technology, yeast artificial chromosome vectors have been designed for efficient clone characterization, manipulation, and mapping. The vectors contain a polylinker with unique EcoRI, BglII, NotI, EagI, SacII, SalI, NruI, NheI, and ClaI cloning sites and T7 bacteriophage promoters positioned to allow the generation of riboprobes from the exogenous DNA ends. Centric and acentric vector arms were constructed as separate plasmids to allow the recovery of both ends of the YAC insert DNA directly in Escherichia coli. In addition, YACs generated using this vector system contain a yeast gene (SUP 11) that allows visual monitoring of YAC stability and copy number.

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Year:  1991        PMID： 2071158     DOI： 10.1016/0888-7543(91)90343-d

Source DB:  PubMed          Journal:  Genomics        ISSN： 0888-7543            Impact factor:   5.736


  8 in total

1.  Construction and characterization of a soybean yeast artificial chromosome library and identification of clones for the Rps6 region.

Authors:  Dipak K Santra; Devinder Sandhu; Thomas Tai; Madan K Bhattacharyya
Journal:  Funct Integr Genomics       Date:  2003-10-01       Impact factor: 3.410

Review 2.  Long-range walking techniques in positional cloning strategies.

Authors:  L Stubbs
Journal:  Mamm Genome       Date:  1992       Impact factor: 2.957

3.  A human DAZ transgene confers partial rescue of the mouse Dazl null phenotype.

Authors:  R Slee; B Grimes; R M Speed; M Taggart; S M Maguire; A Ross; N I McGill; P T Saunders; H J Cooke
Journal:  Proc Natl Acad Sci U S A       Date:  1999-07-06       Impact factor: 11.205

4.  Low-frequency chimeric yeast artificial chromosome libraries from flow-sorted human chromosomes 16 and 21.

Authors:  M K McCormick; E Campbell; L Deaven; R Moyzis
Journal:  Proc Natl Acad Sci U S A       Date:  1993-02-01       Impact factor: 11.205

5.  Specific cloning of human DNA as yeast artificial chromosomes by transformation-associated recombination.

Authors:  V Larionov; N Kouprina; J Graves; X N Chen; J R Korenberg; M A Resnick
Journal:  Proc Natl Acad Sci U S A       Date:  1996-01-09       Impact factor: 11.205

6.  Conservation of fine-scale DNA marker order in the genomes of rice and the Triticeae.

Authors:  R P Dunford; N Kurata; D A Laurie; T A Money; Y Minobe; G Moore
Journal:  Nucleic Acids Res       Date:  1995-07-25       Impact factor: 16.971

Review 7.  Yeast artificial chromosome cloning.

Authors:  M Ramsay
Journal:  Mol Biotechnol       Date:  1994-04       Impact factor: 2.695

8.  A Chlamydomonas genomic library in yeast artificial chromosomes.

Authors:  A Infante; S Lo; J L Hall
Journal:  Genetics       Date:  1995-09       Impact factor: 4.562
  8 in total

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