Literature DB >> 20708035

A pseudo-plaque method for infectious particle assay and clonal isolation of adeno-associated virus.

Daniel A J Mitchell1, Thomas F Lerch, Joan T Hare, Michael S Chapman.   

Abstract

A colorimetric method has been developed for the detection of adeno-associated virus (AAV) infectious centers in cell culture monolayers. Due to its non-cytopathic nature, AAV has not been amenable to the traditional plaque assay, involving an agar overlay and cellular stains. As a result, an alternate method was required. The pseudo-plaque assay is based on enzyme-catalyzed color development after a fixed cell monolayer is probed with anti-AAV monoclonal antibodies. In spite of chemical fixation, expected to damage the viral genomes and particles, infectious particles can be recovered and amplified for the propagation of viral clones.
Copyright © 2010 Elsevier B.V. All rights reserved.

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Year:  2010        PMID： 20708035      PMCID： PMC2981658          DOI： 10.1016/j.jviromet.2010.08.004

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  28 in total

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Authors:  D Grimm; A Kern; M Pawlita; F Ferrari; R Samulski; J Kleinschmidt
Journal:  Gene Ther       Date:  1999-07       Impact factor: 5.250

2.  Factors influencing recombinant adeno-associated virus production.

Authors:  A Salvetti; S Orève; G Chadeuf; D Favre; Y Cherel; P Champion-Arnaud; J David-Ameline; P Moullier
Journal:  Hum Gene Ther       Date:  1998-03-20       Impact factor: 5.695

Review 3.  Production of recombinant adeno-associated virus.

Authors:  G P Gao; J M Wilson; N A Wivel
Journal:  Adv Virus Res       Date:  2000       Impact factor: 9.937

4.  Feasibility of generating adeno-associated virus packaging cell lines containing inducible adenovirus helper genes.

Authors:  Chunping Qiao; Juan Li; Anna Skold; Xudong Zhang; Xiao Xiao
Journal:  J Virol       Date:  2002-02       Impact factor: 5.103

5.  Replication of adeno-associated virus in synchronized cells without the addition of a helper virus.

Authors:  B Yakobson; T Koch; E Winocour
Journal:  J Virol       Date:  1987-04       Impact factor: 5.103

6.  The adeno-associated virus (AAV) Rep protein acts as both a repressor and an activator to regulate AAV transcription during a productive infection.

Authors:  D J Pereira; D M McCarty; N Muzyczka
Journal:  J Virol       Date:  1997-02       Impact factor: 5.103

7.  Large-scale production, purification and crystallization of wild-type adeno-associated virus-2.

Authors:  Qing Xie; Joan Hare; Joy Turnigan; Michael S Chapman
Journal:  J Virol Methods       Date:  2004-12-01       Impact factor: 2.014

8.  Quantification of adeno-associated virus particles and empty capsids by optical density measurement.

Authors:  Jürg M Sommer; Peter H Smith; Sumathy Parthasarathy; Jesse Isaacs; Sharmila Vijay; Jane Kieran; Sharon K Powell; Alan McClelland; J Fraser Wright
Journal:  Mol Ther       Date:  2003-01       Impact factor: 11.454

9.  Chk1 instability is coupled to mitotic cell death of p53-deficient cells in response to virus-induced DNA damage signaling.

Authors:  Jaana Jurvansuu; Michalis Fragkos; Carin Ingemarsdotter; Peter Beard
Journal:  J Mol Biol       Date:  2007-07-03       Impact factor: 5.469

10.  Cloning of infectious adeno-associated virus genomes in bacterial plasmids.

Authors:  C A Laughlin; J D Tratschin; H Coon; B J Carter
Journal:  Gene       Date:  1983-07       Impact factor: 3.688
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  2 in total

1.  Sequence variability in viral genome non-coding regions likely contribute to observed differences in viral replication amongst MARV strains.

Authors:  Jesus A Alonso; Jean L Patterson
Journal:  Virology       Date:  2013-03-16       Impact factor: 3.616

2.  Pseudo-plaque reduction neutralization test (PPRNT) for the measurement of neutralizing antibodies to Crimean-Congo hemorrhagic fever virus.

Authors:  Nurettin Canakoglu; Engin Berber; Mustafa Ertek; Mustafa D Yoruk; Sukru Tonbak; Yusuf Bolat; Munir Aktas; Ahmet Kalkan; Aykut Ozdarendeli
Journal:  Virol J       Date:  2013-01-03       Impact factor: 4.099
  2 in total

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