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Literature DB >> 20703504

TMBP200, a XMAP215 homologue of tobacco BY-2 cells, has an essential role in plant mitosis.

Abstract

TMBP200 from tobacco BY-2 cells is a member of the highly conserved family of microtubule-associated proteins that includes Xenopus XMAP215, human TOGp, and Arabidopsis MOR1/GEM1. XMAP215 homologues have an essential role in spindle assembly and function in animals and yeast, but their role in plant mitosis is not fully clarified. Here, we show by immunoblot analysis that TMBP200 levels in synchronously cultured BY-2 cells increased when the cells entered mitosis, thus indicating that TMBP200 plays an important role in mitosis in tobacco. To investigate the role of TMBP200 in mitosis, we employed inducible RNA interference to silence TMBP200 expression in BY-2 cells. The resulting depletion of TMBP200 caused severe defects in bipolar spindle formation and resulted in the appearance of multinucleated cells with variable-sized nuclei. This finding indicates that TMBP200 has an essential role in bipolar spindle formation and function.

Entities: Chemical Disease Gene Species

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Year: 2010 PMID： 20703504 DOI： 10.1007/s00709-010-0189-6

Source DB: PubMed Journal: Protoplasma ISSN： 0033-183X Impact factor: 3.356

27 in total

1. The 65-kDa carrot microtubule-associated protein forms regularly arranged filamentous cross-bridges between microtubules.

Authors: J Chan; C G Jensen; L C Jensen; M Bush; C W Lloyd
Journal: Proc Natl Acad Sci U S A Date: 1999-12-21 Impact factor: 11.205

2. Fate of nascent microtubules organized at the M/G1 interface, as visualized by synchronized tobacco BY-2 cells stably expressing GFP-tubulin: time-sequence observations of the reorganization of cortical microtubules in living plant cells.

Authors: F Kumagai; A Yoneda; T Tomida; T Sano; T Nagata; S Hasezawa
Journal: Plant Cell Physiol Date: 2001-07 Impact factor: 4.927

3. Differential functional interplay of TOGp/XMAP215 and the KinI kinesin MCAK during interphase and mitosis.

Authors: Per Holmfeldt; Sonja Stenmark; Martin Gullberg
Journal: EMBO J Date: 2004-01-29 Impact factor: 11.598

4. TOGp, the human homolog of XMAP215/Dis1, is required for centrosome integrity, spindle pole organization, and bipolar spindle assembly.

Authors: Lynne Cassimeris; Justin Morabito
Journal: Mol Biol Cell Date: 2004-01-12 Impact factor: 4.138

5. MICROTUBULE ORGANIZATION 1 regulates structure and function of microtubule arrays during mitosis and cytokinesis in the Arabidopsis root.

Authors: Eiko Kawamura; Regina Himmelspach; Madeleine C Rashbrooke; Angela T Whittington; Kevin R Gale; David A Collings; Geoffrey O Wasteneys
Journal: Plant Physiol Date: 2005-12-23 Impact factor: 8.340

6. Inhibition of cell-plate formation by brefeldin A inhibited the depolymerization of microtubules in the central region of the phragmoplast.

Authors: H Yasuhara; H Shibaoka
Journal: Plant Cell Physiol Date: 2000-03 Impact factor: 4.927

Review 7. MAPping the eukaryotic tree of life: structure, function, and evolution of the MAP215/Dis1 family of microtubule-associated proteins.

Authors: David L Gard; Bret E Becker; S Josh Romney
Journal: Int Rev Cytol Date: 2004

8. MOR1, the Arabidopsis thaliana homologue of Xenopus MAP215, promotes rapid growth and shrinkage, and suppresses the pausing of microtubules in vivo.

Authors: Eiko Kawamura; Geoffrey O Wasteneys
Journal: J Cell Sci Date: 2008-11-25 Impact factor: 5.285

TMBP200, a XMAP215 homologue of tobacco BY-2 cells, has an essential role in plant mitosis.

1. The 65-kDa carrot microtubule-associated protein forms regularly arranged filamentous cross-bridges between microtubules.

2. Fate of nascent microtubules organized at the M/G1 interface, as visualized by synchronized tobacco BY-2 cells stably expressing GFP-tubulin: time-sequence observations of the reorganization of cortical microtubules in living plant cells.

3. Differential functional interplay of TOGp/XMAP215 and the KinI kinesin MCAK during interphase and mitosis.

4. TOGp, the human homolog of XMAP215/Dis1, is required for centrosome integrity, spindle pole organization, and bipolar spindle assembly.

5. MICROTUBULE ORGANIZATION 1 regulates structure and function of microtubule arrays during mitosis and cytokinesis in the Arabidopsis root.

6. Inhibition of cell-plate formation by brefeldin A inhibited the depolymerization of microtubules in the central region of the phragmoplast.

Review 7. MAPping the eukaryotic tree of life: structure, function, and evolution of the MAP215/Dis1 family of microtubule-associated proteins.

8. MOR1, the Arabidopsis thaliana homologue of Xenopus MAP215, promotes rapid growth and shrinkage, and suppresses the pausing of microtubules in vivo.

9. The TOGp protein is a new human microtubule-associated protein homologous to the Xenopus XMAP215.

10. Identification of XMAP215 as a microtubule-destabilizing factor in Xenopus egg extract by biochemical purification.

1. The same, but different--a bird's-eye view on mitosis.

2. Cytoplasmic MTOCs control spindle orientation for asymmetric cell division in plants.

Review 3. The functions of the cytoskeleton and associated proteins during mitosis and cytokinesis in plant cells.

4. TOG-tubulin binding specificity promotes microtubule dynamics and mitotic spindle formation.