AIM: Reactivation of latent BK polyomavirus (BKV) infection is relatively common following renal transplantation and BKV-associated nephropathy has emerged as a significant complication. JC polyomavirus (JCV) reactivation is less well studied. The aim of the study was to determine reactivation patterns for these polyomaviruses in renal transplant recipients using an in-house quantitative real-time multiplex PCR assay and IgG serological assays using recombinant BK and JC virus-like particles. METHODS: Retrospective analysis of urine and plasma samples collected from 30 renal transplant patients from February 2004 to May 2005 at Leeds Teaching Hospitals NHS Trust. Samples were collected at 5 days and thereafter at 1, 3, 6 and 12 months post-transplantation. RESULTS: Eight patients (26.7%) were positive for BK viruria; three of these patients submitted plasma samples and two had BK viraemia. Five patients (16.7%) were positive for JC viruria. A corresponding rise in BKV and JCV antibody titres was seen in association with high levels of viruria. CONCLUSIONS: Different patterns of reactivation were observed: BK viruria was detected after 3-6 months, and JC viruria was observed as early as 5 days post-transplantation. One patient had biopsy-proven BKV nephropathy. No dual infections were seen. In order to ensure better graft survival, early diagnosis of these polyomaviruses is desirable.
AIM: Reactivation of latent BK polyomavirus (BKV) infection is relatively common following renal transplantation and BKV-associated nephropathy has emerged as a significant complication. JC polyomavirus (JCV) reactivation is less well studied. The aim of the study was to determine reactivation patterns for these polyomaviruses in renal transplant recipients using an in-house quantitative real-time multiplex PCR assay and IgG serological assays using recombinant BK and JC virus-like particles. METHODS: Retrospective analysis of urine and plasma samples collected from 30 renal transplant patients from February 2004 to May 2005 at Leeds Teaching Hospitals NHS Trust. Samples were collected at 5 days and thereafter at 1, 3, 6 and 12 months post-transplantation. RESULTS: Eight patients (26.7%) were positive for BK viruria; three of these patients submitted plasma samples and two had BK viraemia. Five patients (16.7%) were positive for JC viruria. A corresponding rise in BKV and JCV antibody titres was seen in association with high levels of viruria. CONCLUSIONS: Different patterns of reactivation were observed: BK viruria was detected after 3-6 months, and JC viruria was observed as early as 5 days post-transplantation. One patient had biopsy-proven BKV nephropathy. No dual infections were seen. In order to ensure better graft survival, early diagnosis of these polyomaviruses is desirable.
Authors: Dana E Rollison; Anna R Giuliano; Jane L Messina; Neil A Fenske; Basil S Cherpelis; Vernon K Sondak; Richard G Roetzheim; Michelle R Iannacone; Kristina M Michael; Tarik Gheit; Tim Waterboer; Massimo Tommasino; Michael Pawlita Journal: Cancer Epidemiol Biomarkers Prev Date: 2011-10-20 Impact factor: 4.254
Authors: Anala Gossai; Tim Waterboer; Heather H Nelson; Jennifer A Doherty; Angelika Michel; Martina Willhauck-Fleckenstein; Shohreh F Farzan; Brock C Christensen; Anne G Hoen; Ann E Perry; Michael Pawlita; Margaret R Karagas Journal: Cancer Epidemiol Biomarkers Prev Date: 2016-02-11 Impact factor: 4.254
Authors: Jasmin Divers; Marina Núñez; Kevin P High; Mariana Murea; Michael V Rocco; Lijun Ma; Donald W Bowden; Pamela J Hicks; Mitzie Spainhour; David A Ornelles; Steven B Kleiboeker; Kara Duncan; Carl D Langefeld; Jolyn Turner; Barry I Freedman Journal: Kidney Int Date: 2013-05-15 Impact factor: 10.612
Authors: Anala Gossai; Tim Waterboer; Anne G Hoen; Shohreh F Farzan; Heather H Nelson; Angelika Michel; Martina Willhauck-Fleckenstein; Brock C Christensen; Ann E Perry; Michael Pawlita; Margaret R Karagas Journal: Cancer Med Date: 2016-02-21 Impact factor: 4.452
Authors: Darlene Vigil; Nikifor K Konstantinov; Marc Barry; Antonia M Harford; Karen S Servilla; Young Ho Kim; Yijuan Sun; Kavitha Ganta; Antonios H Tzamaloukas Journal: World J Transplant Date: 2016-09-24