BACKGROUND: Rhesus monkeys play a central role in model studies on human infectious diseases, and often mucosal organs are affected by these pathogens, e.g. HIV. However, a comparative investigation into lymphocyte composition from different mucosal tissues is still missing. METHODS: Lymphocyte composition of duodenum, jejunum, ileum, colon, vagina, cervix, uterus and bronchoalveolar lavage from healthy rhesus monkeys was characterized in detail by flow cytometry. Moreover, we compared the lymphocyte proportions from intestinal biopsies with resections. RESULTS: All mucosal tissues exhibited higher values of CD8(+) , CD4(+) CCR5(+) and CD45RA(-) memory T cells than blood, but similar levels of total T cells. Especially within the four gut sites, the lymphocyte composition varied significantly. The relative proportions of lymphocyte subsets from duodenal and colonic biopsies compared to resections differed. CONCLUSION: The lymphocyte composition highly varies between different mucosal sites, and data obtained from biopsy and necropsy samples were mostly not comparable.
BACKGROUND:Rhesus monkeys play a central role in model studies on humaninfectious diseases, and often mucosal organs are affected by these pathogens, e.g. HIV. However, a comparative investigation into lymphocyte composition from different mucosal tissues is still missing. METHODS: Lymphocyte composition of duodenum, jejunum, ileum, colon, vagina, cervix, uterus and bronchoalveolar lavage from healthy rhesus monkeys was characterized in detail by flow cytometry. Moreover, we compared the lymphocyte proportions from intestinal biopsies with resections. RESULTS: All mucosal tissues exhibited higher values of CD8(+) , CD4(+) CCR5(+) and CD45RA(-) memory T cells than blood, but similar levels of total T cells. Especially within the four gut sites, the lymphocyte composition varied significantly. The relative proportions of lymphocyte subsets from duodenal and colonic biopsies compared to resections differed. CONCLUSION: The lymphocyte composition highly varies between different mucosal sites, and data obtained from biopsy and necropsy samples were mostly not comparable.
Authors: Michael A Thomas; Thorsten Demberg; Diego A Vargas-Inchaustegui; Peng Xiao; Iskra Tuero; David Venzon; Deborah Weiss; James Treece; Marjorie Robert-Guroff Journal: Vaccine Date: 2013-12-25 Impact factor: 3.641
Authors: Sarah Brinckmann; Kelly da Costa; Marit J van Gils; David Hallengärd; Katja Klein; Luisa Madeira; Lara Mainetti; Paolo Palma; Katharina Raue; David Reinhart; Marc Reudelsterz; Nicolas Ruffin; Janna Seifried; Katrein Schäfer; Enas Sheik-Khalil; Annette Sköld; Hannes Uchtenhagen; Nicolas Vabret; Serena Ziglio; Gabriella Scarlatti; Robin Shattock; Britta Wahren; Frances Gotch Journal: J Transl Med Date: 2011-04-12 Impact factor: 5.531
Authors: Simone Joas; Erica H Parrish; Clement W Gnanadurai; Edina Lump; Christina M Stürzel; Nicholas F Parrish; Gerald H Learn; Ulrike Sauermann; Berit Neumann; Kerstin Mätz Rensing; Dietmar Fuchs; James M Billingsley; Steven E Bosinger; Guido Silvestri; Cristian Apetrei; Nicolas Huot; Thalia Garcia-Tellez; Michaela Müller-Trutwin; Dominik Hotter; Daniel Sauter; Christiane Stahl-Hennig; Beatrice H Hahn; Frank Kirchhoff Journal: Nat Commun Date: 2018-04-10 Impact factor: 14.919