Literature DB >> 206966

Reversed-phase, high-pressure liquid chromatography of peptides and proteins with ion-pairing reagents.

W S Hancock, C A Bishop, R L Prestidge, D R Harding, M T Hearn.   

Abstract

Reversed-phase, high-pressure liquid chromatography has been successfully applied to the analysis of peptides and proteins by the addition of hydrophilic (for example, phosphoric acid) or hydrophobic (for example, hexanesulfonic acid) ion-pairing reagents, or both, to the mobile phase. Examples described included proteins such as insulin, glucagon, and 1-24 ACTH pentaacetate (ACTH is adrenocorticotrophic hormone).

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Year:  1978        PMID： 206966     DOI： 10.1126/science.206966

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  8 in total

1.  Heterogeneity of CD4-positive human T-cell clones which recognize the surface protein antigen of Rickettsia typhi.

Authors:  M Carl; S Vaidya; F M Robbins; W M Ching; R J Hartzman; G A Dasch
Journal:  Infect Immun       Date:  1989-04       Impact factor: 3.441

2.  Prediction of peptide retention times in high-pressure liquid chromatography on the basis of amino acid composition.

Authors:  J L Meek
Journal:  Proc Natl Acad Sci U S A       Date:  1980-03       Impact factor: 11.205

3.  Separation of the apoprotein components of human very low density lipoproteins by ion-paired, reversed-phase high performance liquid chromatography.

Authors:  W S Hancock; C A Bishop; A M Gotto; D R Harding; S M Lamplugh; J T Sparrow
Journal:  Lipids       Date:  1981-04       Impact factor: 1.880

4.  Two forms of murine epidermal growth factor: rapid separation by using reverse-phase HPLC.

Authors:  A W Burgess; J Knesel; L G Sparrow; N A Nicola; E C Nice
Journal:  Proc Natl Acad Sci U S A       Date:  1982-10       Impact factor: 11.205

5.  Optimization of reversed-phase peptide liquid chromatography ultraviolet mass spectrometry analyses using an automated blending methodology.

Authors:  Asish B Chakraborty; Scott J Berger
Journal:  J Biomol Tech       Date:  2005-12

6.  Reverse-phase HPLC of DNA restriction fragments and ribooligonucleotides on uncoated Kel-F powder.

Authors:  D A Usher
Journal:  Nucleic Acids Res       Date:  1979       Impact factor: 16.971

7.  Identification and quantification of glycoproteins using ion-pairing normal-phase liquid chromatography and mass spectrometry.

Authors:  Wen Ding; Harald Nothaft; Christine M Szymanski; John Kelly
Journal:  Mol Cell Proteomics       Date:  2009-06-12       Impact factor: 5.911

8.  Reversed-phase fused-core HPLC modeling of peptides.

Authors:  Matthias D'Hondt; Bert Gevaert; Sofie Stalmans; Sylvia Van Dorpe; Evelien Wynendaele; Kathelijne Peremans; Christian Burvenich; Bart De Spiegeleer
Journal:  J Pharm Anal       Date:  2012-11-30
  8 in total

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