Literature DB >> 20693665

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of SAICAR synthase from Streptococcus suis serotype 2.

Xia Cheng1, Guangwen Lu, Jianxun Qi, Hao Cheng, Feng Gao, Jundong Wang, Jinghua Yan.   

Abstract

Phosphoribosylaminoimidazole-succinocarboxamide synthase (SAICAR synthase) plays an essential role in the de novo biosynthesis of purine nucleotides. In this study, the SAICAR synthase from Streptococcus suis was cloned and overexpressed in Escherichia coli. The subsequent product was purified and crystallized using the hanging-drop vapour-diffusion method. The crystals diffracted to 2.8 A resolution and belonged to space group P2, with unit-cell parameters a=70.2, b=52.2, c=153.9 A, beta=102.8 degrees.

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Year:  2010        PMID： 20693665      PMCID： PMC2917288          DOI： 10.1107/S1744309110020518

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  9 in total

1.  The structure of SAICAR synthase: an enzyme in the de novo pathway of purine nucleotide biosynthesis.

Authors:  V M Levdikov; V V Barynin; A I Grebenko; W R Melik-Adamyan; V S Lamzin; K S Wilson
Journal:  Structure       Date:  1998-03-15       Impact factor: 5.006

2.  Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of the putative SAICAR synthetase (PH0239) from Pyrococcus horikoshii OT3.

Authors:  Kavyashree Manjunath; Jeyaraman Jeyakanthan; Noriko Nakagawa; Akeo Shinkai; Masato Yoshimura; Seiki Kuramitsu; Shigeyuki Yokoyama; Kanagaraj Sekar
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2010-01-28

3.  Nucleotide complexes of Escherichia coli phosphoribosylaminoimidazole succinocarboxamide synthetase.

Authors:  Nathaniel D Ginder; Daniel J Binkowski; Herbert J Fromm; Richard B Honzatko
Journal:  J Biol Chem       Date:  2006-05-09       Impact factor: 5.157

Review 4.  The pathogenesis of the meningitis caused by Streptococcus suis: the unresolved questions.

Authors:  M Gottschalk; M Segura
Journal:  Vet Microbiol       Date:  2000-10-01       Impact factor: 3.293

Review 5.  Streptococcus suis: past and present.

Authors:  J J Staats; I Feder; O Okwumabua; M M Chengappa
Journal:  Vet Res Commun       Date:  1997-08       Impact factor: 2.459

6.  Human Streptococcus suis outbreak, Sichuan, China.

Authors:  Hongjie Yu; Huaiqi Jing; Zhihai Chen; Han Zheng; Xiaoping Zhu; Hua Wang; Shiwen Wang; Lunguang Liu; Rongqiang Zu; Longze Luo; Nijuan Xiang; Honglu Liu; Xuecheng Liu; Yuelong Shu; Shui Shan Lee; Shuk Kwan Chuang; Yu Wang; Jianguo Xu; Weizhong Yang
Journal:  Emerg Infect Dis       Date:  2006-06       Impact factor: 6.883

7.  A glimpse of streptococcal toxic shock syndrome from comparative genomics of S. suis 2 Chinese isolates.

Authors:  Chen Chen; Jiaqi Tang; Wei Dong; Changjun Wang; Youjun Feng; Jing Wang; Feng Zheng; Xiuzhen Pan; Di Liu; Ming Li; Yajun Song; Xinxing Zhu; Haibo Sun; Tao Feng; Zhaobiao Guo; Aiping Ju; Junchao Ge; Yaqing Dong; Wen Sun; Yongqiang Jiang; Jun Wang; Jinghua Yan; Huanming Yang; Xiaoning Wang; George F Gao; Ruifu Yang; Jian Wang; Jun Yu
Journal:  PLoS One       Date:  2007-03-21       Impact factor: 3.240

8.  Structure of SAICAR synthase from Thermotoga maritima at 2.2 angstroms reveals an unusual covalent dimer.

Authors:  Rongguang Zhang; Tatiana Skarina; Elena Evdokimova; Aled Edwards; Alexei Savchenko; Roman Laskowski; Marianne E Cuff; Andrzej Joachimiak
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2006-03-25

9.  Streptococcal toxic shock syndrome caused by Streptococcus suis serotype 2.

Authors:  Jiaqi Tang; Changjun Wang; Youjun Feng; Weizhong Yang; Huaidong Song; Zhihai Chen; Hongjie Yu; Xiuzhen Pan; Xiaojun Zhou; Huaru Wang; Bo Wu; Haili Wang; Huamei Zhao; Ying Lin; Jianhua Yue; Zhenqiang Wu; Xiaowei He; Feng Gao; Abdul Hamid Khan; Jian Wang; Guo-Ping Zhao; Yu Wang; Xiaoning Wang; Zhu Chen; George F Gao
Journal:  PLoS Med       Date:  2006-04-11       Impact factor: 11.069
  9 in total

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