OBJECTIVE: The objective of the study was to isolate and characterize a population of mesenchymal stem cells (MSCs) from human term placental membranes. STUDY DESIGN: We isolated an adherent cell population from extraembryonic membranes. Morphology, phenotype, growth characteristics, karyotype, and immunological and differentiation properties were analyzed. RESULTS: The isolated placental MSCs were from maternal origin and named as decidua-derived mesenchymal stem cells (DMSCs). DMSCs differentiated into derivatives of all germ layers. It is the first report about placental MSC differentiation into alveolar type II cells. Clonally expanded DMSCs differentiated into all embryonic layers, including pulmonary cells. DMSCs showed higher life span than placental cells from fetal origin and proliferated without genomic instability. CONCLUSION: The data suggest that DMSCs are true multipotent MSCs, distinguishing them from other placental MSCs. DMSCs could be safely used in the mother as a potential source of MSCs for pelvic floor dysfunctions and immunological diseases. Additionally, frozen DMSCs can be stored for both autologous and allogeneic tissue regeneration.
OBJECTIVE: The objective of the study was to isolate and characterize a population of mesenchymal stem cells (MSCs) from human term placental membranes. STUDY DESIGN: We isolated an adherent cell population from extraembryonic membranes. Morphology, phenotype, growth characteristics, karyotype, and immunological and differentiation properties were analyzed. RESULTS: The isolated placental MSCs were from maternal origin and named as decidua-derived mesenchymal stem cells (DMSCs). DMSCs differentiated into derivatives of all germ layers. It is the first report about placental MSC differentiation into alveolar type II cells. Clonally expanded DMSCs differentiated into all embryonic layers, including pulmonary cells. DMSCs showed higher life span than placental cells from fetal origin and proliferated without genomic instability. CONCLUSION: The data suggest that DMSCs are true multipotent MSCs, distinguishing them from other placental MSCs. DMSCs could be safely used in the mother as a potential source of MSCs for pelvic floor dysfunctions and immunological diseases. Additionally, frozen DMSCs can be stored for both autologous and allogeneic tissue regeneration.
Authors: Jennifer M Ryan; Allison R Pettit; Pascale V Guillot; Jerry K Y Chan; Nicholas M Fisk Journal: Stem Cell Rev Rep Date: 2013-08 Impact factor: 5.739
Authors: Celena F Heazlewood; Helen Sherrell; Jennifer Ryan; Kerry Atkinson; Christine A Wells; Nicholas M Fisk Journal: Stem Cells Transl Med Date: 2014-08-25 Impact factor: 6.940
Authors: María Del Pilar Martínez-Montiel; Gonzalo Jesús Gómez-Gómez; Ana Isabel Flores Journal: World J Gastroenterol Date: 2014-02-07 Impact factor: 5.742