BACKGROUND: Portal vein embolization (PVE) is a technique to increase future remnant liver volume. A standardized animal model, resembling the clinical PVE procedure, is needed to clarify some of the unresolved issues surrounding PVE. For this purpose we developed a new rabbit model for PVE. MATERIALS AND METHODS: Twenty female New Zealand white rabbits were allocated to two protocols, each containing two subgroups. Eighty percent of the liver portal venous system was embolized with polyvinyl-alcohol particles and coils (protocol 1: 300-500 μm particles and one coil; protocol 2: 90-180 μm combined 300-500 μm particles and three coils). In all rabbits CT-volumetry, ICG clearance test, blood sampling, and portography were performed prior to PVE and at d 7 and 14. Additional blood sampling and CT volumetry was done on d 3 and 7. RESULTS: PVE was technically feasible in the rabbit. CT-volumetry demonstrated a strong correlation with actual liver weight and volume measured at sacrifice. The hypertrophy response was highest at d 7 in both protocols, which was consistent with the amount of proliferating hepatocytes. Protocol 2 showed less revascularization of the portal venous system and demonstrated the highest hypertrophy response. Comparable to the clinical situation, only a small, transient increase in transaminases was observed. There were no changes in liver function parameters after PVE. Histopathologic findings in the rabbit livers were comparable to those found in human livers. CONCLUSIONS: We successfully devised a rabbit model for PVE, which resembles the human clinical situation.
BACKGROUND: Portal vein embolization (PVE) is a technique to increase future remnant liver volume. A standardized animal model, resembling the clinical PVE procedure, is needed to clarify some of the unresolved issues surrounding PVE. For this purpose we developed a new rabbit model for PVE. MATERIALS AND METHODS: Twenty female New Zealand white rabbits were allocated to two protocols, each containing two subgroups. Eighty percent of the liver portal venous system was embolized with polyvinyl-alcohol particles and coils (protocol 1: 300-500 μm particles and one coil; protocol 2: 90-180 μm combined 300-500 μm particles and three coils). In all rabbits CT-volumetry, ICG clearance test, blood sampling, and portography were performed prior to PVE and at d 7 and 14. Additional blood sampling and CT volumetry was done on d 3 and 7. RESULTS: PVE was technically feasible in the rabbit. CT-volumetry demonstrated a strong correlation with actual liver weight and volume measured at sacrifice. The hypertrophy response was highest at d 7 in both protocols, which was consistent with the amount of proliferating hepatocytes. Protocol 2 showed less revascularization of the portal venous system and demonstrated the highest hypertrophy response. Comparable to the clinical situation, only a small, transient increase in transaminases was observed. There were no changes in liver function parameters after PVE. Histopathologic findings in the rabbit livers were comparable to those found in human livers. CONCLUSIONS: We successfully devised a rabbit model for PVE, which resembles the human clinical situation.
Authors: Andrew C Gordon; Sarah B White; Vanessa L Gates; Weiguo Li; Daniel Procissi; Zhuoli Zhang; Kathleen R Harris; Dong-Hyun Kim; Samdeep K Mouli; Reed A Omary; Riad Salem; Andrew C Larson; Robert J Lewandowski Journal: Cardiovasc Intervent Radiol Date: 2020-08-16 Impact factor: 2.740
Authors: Jaydev K Dave; Ji-Bin Liu; Valgerdur G Halldorsdottir; John R Eisenbrey; Daniel A Merton; Priscilla Machado; Hongjia Zhao; Joseph Altemus; Laurence Needleman; Daniel B Brown; Flemming Forsberg Journal: Comp Med Date: 2012-10 Impact factor: 0.982
Authors: Chris M van der Loos; Onno J de Boer; Claire Mackaaij; Lisette T Hoekstra; Thomas M van Gulik; Joanne Verheij Journal: J Histochem Cytochem Date: 2012-09-01 Impact factor: 2.479
Authors: María Páramo; Paula García-Barquin; Eva Santa María; José Miguel Madrid; Meylin Caballeros; Alberto Benito; Bruno Sangro; Mercedes Iñarrairaegui; José Ignacio Bilbao Journal: Eur Radiol Exp Date: 2017-06-29
Authors: María Páramo; Eva Santamaría; Miguel A Idoate; Macarena Rodríguez-Fraile; Alberto Benito; Maria Collantes; Gemma Quincoces; Iván Peñuelas; Carmen Berasain; Josepmaria Argemi; Jorge Quiroga; Bruno Sangro; José I Bilbao; Mercedes Iñarrairaegui Journal: Sci Rep Date: 2022-02-02 Impact factor: 4.379