OBJECTIVE: A genome-wide association study and a replication using Japanese, Spanish and Greek Caucasian populations have recently indicated two single-nucleotide polymorphisms (SNPs) (rs7775228 and rs10947262) associated with knee Osteoarthritis (OA) susceptibility. We have further evaluated the association in knee OA subjects from Han Chinese and Australian Caucasian origin. METHODS: Two independent case-control association studies were performed using Han Chinese and Australian Caucasian populations. The two SNPs were genotyped in patients who had primary symptomatic knee OA with radiographic confirmation and/or received total knee replacement surgery as well as in matched controls. They were subjected to statistic analyses. RESULTS: A total of 991 OA patients and 1536 controls were genotyped. No significant difference was detected in genotype or allele frequencies of the two SNPs between knee OA and control groups in the two populations (all P>0.05). The association was also negative even after stratification by sex, body mass index (BMI) and Kellgren/Lawrence scores. The significant heterogeneity was detected between Chinese and Japanese (both P<0.05). In the Caucasian samples, no significant heterogeneity was detected (both P>0.05). The result of meta-analysis showed significant association between knee OA and rs10947262 in total subjects [summary OR=1.26, 95%confidence intervals (CI)=1.07-1.27, P=3 × 10(-8)] and in Caucasian samples (summary OR=1.28, 95%CI=1.04-1.57, P=0.02). CONCLUSION: We demonstrated no association between the two SNPs in human leukocyte antigen (HLA) class II/III region and knee OA in Han Chinese population. A significant association was detected between SNP rs10947262 and knee OA in Caucasian subjects. Further replication studies are required to identify the impact of controversial association.
OBJECTIVE: A genome-wide association study and a replication using Japanese, Spanish and Greek Caucasian populations have recently indicated two single-nucleotide polymorphisms (SNPs) (rs7775228 and rs10947262) associated with knee Osteoarthritis (OA) susceptibility. We have further evaluated the association in knee OA subjects from Han Chinese and Australian Caucasian origin. METHODS: Two independent case-control association studies were performed using Han Chinese and Australian Caucasian populations. The two SNPs were genotyped in patients who had primary symptomatic knee OA with radiographic confirmation and/or received total knee replacement surgery as well as in matched controls. They were subjected to statistic analyses. RESULTS: A total of 991 OA patients and 1536 controls were genotyped. No significant difference was detected in genotype or allele frequencies of the two SNPs between knee OA and control groups in the two populations (all P>0.05). The association was also negative even after stratification by sex, body mass index (BMI) and Kellgren/Lawrence scores. The significant heterogeneity was detected between Chinese and Japanese (both P<0.05). In the Caucasian samples, no significant heterogeneity was detected (both P>0.05). The result of meta-analysis showed significant association between knee OA and rs10947262 in total subjects [summary OR=1.26, 95%confidence intervals (CI)=1.07-1.27, P=3 × 10(-8)] and in Caucasian samples (summary OR=1.28, 95%CI=1.04-1.57, P=0.02). CONCLUSION: We demonstrated no association between the two SNPs in human leukocyte antigen (HLA) class II/III region and knee OA in Han Chinese population. A significant association was detected between SNP rs10947262 and knee OA in Caucasian subjects. Further replication studies are required to identify the impact of controversial association.
Authors: Ana M Valdes; Unnur Styrkarsdottir; Michael Doherty; David L Morris; Massimo Mangino; Agu Tamm; Sally A Doherty; Kalle Kisand; Irina Kerna; Ann Tamm; Margaret Wheeler; Rose A Maciewicz; Weiya Zhang; Kenneth R Muir; Elaine M Dennison; Deborah J Hart; Sarah Metrustry; Ingileif Jonsdottir; Gudbjorn F Jonsson; Helgi Jonsson; Thorvaldur Ingvarsson; Cyrus Cooper; Timothy J Vyse; Tim D Spector; Kari Stefansson; Nigel K Arden Journal: PLoS One Date: 2011-08-10 Impact factor: 3.240
Authors: Susanna Lemmelä; Svetlana Solovieva; Rahman Shiri; Christian Benner; Markku Heliövaara; Johannes Kettunen; Verneri Anttila; Samuli Ripatti; Markus Perola; Ilkka Seppälä; Markus Juonala; Mika Kähönen; Veikko Salomaa; Jorma Viikari; Olli T Raitakari; Terho Lehtimäki; Aarno Palotie; Eira Viikari-Juntura; Kirsti Husgafvel-Pursiainen Journal: PLoS One Date: 2016-10-20 Impact factor: 3.240
Authors: Lin Han; Joo Hyoun Song; Jung Hwan Yoon; Yong Gyu Park; Suk Woo Lee; Yoo Jin Choi; Suk Woo Nam; Jung Young Lee; Won Sang Park Journal: Korean J Pathol Date: 2012-02-23