| Literature DB >> 20691216 |
Chuanying Pan1, Xianyong Lan, Hong Chen, Colin E Bishop.
Abstract
A simple, single-sided antibody method for incubating primary and secondary antibodies in Western blotting was developed, which generates significant savings on the use of antibodies. Compared with the conventional immersion technique for antibody incubation, the present economical single-sided antibody incubation method resulted in a saving of 80% of antibody use. Besides, the present incubation method did not compromise the Western blot results and was not affected by the expression levels of target proteins.Mesh：
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Year: 2010 PMID： 20691216 PMCID： PMC8330846 DOI： 10.1016/j.jviromet.2010.07.026
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014