Literature DB >> 20686769

The novel responses of ethambutol against Mycobacterium smegmatis mc²155 Revealed by proteomics analysis.

Tao Jiang1, Yaoyao Zhan, Mingzhong Sun, Shuqing Liu, Shizhu Zang, Yufang Ma, Yi Xin.   

Abstract

Ethambutol (EMB), one of the effective anti-mycobacterial drugs, inhibits the biosynthesis of mycobacterium cell wall. To elucidate the molecular mechanism of EMB against tuberculosis (TB), Mycobacterium smegmatis mc²155 was employed as a model of mycobacterial system in this study. We compared the protein profiles on M. smegmatis mc²155 treated by EMB and untreated using fluorescence difference two-dimensional gel electrophoresis (2-D DIGE). A total of 40 differential protein spots were selected and 22 proteins were identified by HPLC-nano ESI-MS/MS analysis, including 16 over-expressed proteins and 6 under-expressed proteins. These proteins mainly affected energy metabolism, as well as synthesis and modification of macromolecules. The expressions of correspondent genes were confirmed by RT-PCR. This investigation provided some clues for searching potential drug targets.

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Year:  2010        PMID： 20686769     DOI： 10.1007/s00284-010-9711-5

Source DB:  PubMed          Journal:  Curr Microbiol        ISSN： 0343-8651            Impact factor:   2.188


  16 in total

1.  The transcriptional responses of Mycobacterium tuberculosis to inhibitors of metabolism: novel insights into drug mechanisms of action.

Authors:  Helena I M Boshoff; Timothy G Myers; Brent R Copp; Michael R McNeil; Michael A Wilson; Clifton E Barry
Journal:  J Biol Chem       Date:  2004-07-09       Impact factor: 5.157

2.  Crystal structures of mycolic acid cyclopropane synthases from Mycobacterium tuberculosis.

Authors:  Chih-chin Huang; Clare V Smith; Michael S Glickman; William R Jacobs; James C Sacchettini
Journal:  J Biol Chem       Date:  2001-12-26       Impact factor: 5.157

3.  Capabilities using 2-D DIGE in proteomics research : the new gold standard for 2-D gel electrophoresis.

Authors:  Christine R Rozanas; Stacey M Loyland
Journal:  Methods Mol Biol       Date:  2008

4.  Proteomics analysis of two mice hepatocarcinoma ascites syngeneic cell lines with high and low lymph node metastasis rates provide potential protein markers for tumor malignancy attributes to lymphatic metastasis.

Authors:  Ming-Zhong Sun; Shuqing Liu; Jianwu Tang; Zhiqiang Wang; Xiaolin Gong; Chengrong Sun; Frederic Greenaway
Journal:  Proteomics       Date:  2009-06       Impact factor: 3.984

5.  The emb operon, a gene cluster of Mycobacterium tuberculosis involved in resistance to ethambutol.

Authors:  A Telenti; W J Philipp; S Sreevatsan; C Bernasconi; K E Stockbauer; B Wieles; J M Musser; W R Jacobs
Journal:  Nat Med       Date:  1997-05       Impact factor: 53.440

6.  Mycobacterium tuberculosis expresses two chaperonin-60 homologs.

Authors:  T H Kong; A R Coates; P D Butcher; C J Hickman; T M Shinnick
Journal:  Proc Natl Acad Sci U S A       Date:  1993-04-01       Impact factor: 11.205

7.  Biogenesis of the mycobacterial cell wall and the site of action of ethambutol.

Authors:  K Mikusová; R A Slayden; G S Besra; P J Brennan
Journal:  Antimicrob Agents Chemother       Date:  1995-11       Impact factor: 5.191

8.  Mutations at embB codon 306 are an important molecular indicator of ethambutol resistance in Mycobacterium tuberculosis.

Authors:  Angela M Starks; Aysel Gumusboga; Bonnie B Plikaytis; Thomas M Shinnick; James E Posey
Journal:  Antimicrob Agents Chemother       Date:  2008-12-22       Impact factor: 5.191

9.  Recognition of multiple effects of ethambutol on metabolism of mycobacterial cell envelope.

Authors:  L Deng; K Mikusová; K G Robuck; M Scherman; P J Brennan; M R McNeil
Journal:  Antimicrob Agents Chemother       Date:  1995-03       Impact factor: 5.191

10.  Characterisation of methionine adenosyltransferase from Mycobacterium smegmatis and M. tuberculosis.

Authors:  Bradley J Berger; Marvin H Knodel
Journal:  BMC Microbiol       Date:  2003-06-16       Impact factor: 3.605
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  5 in total

1.  Avoiding acidic region streaking in two-dimensional gel electrophoresis: case study with two bacterial whole cell protein extracts.

Authors:  Arnab Roy; Umesh Varshney; Debnath Pal
Journal:  J Biosci       Date:  2014-09       Impact factor: 1.826

Review 2.  Energy metabolism and drug efflux in Mycobacterium tuberculosis.

Authors:  Philippa A Black; Robin M Warren; Gail E Louw; Paul D van Helden; Thomas C Victor; Bavesh D Kana
Journal:  Antimicrob Agents Chemother       Date:  2014-03-10       Impact factor: 5.191

3.  Aptamer against mannose-capped lipoarabinomannan inhibits virulent Mycobacterium tuberculosis infection in mice and rhesus monkeys.

Authors:  Qin Pan; Qilong Wang; Xiaoming Sun; Xianru Xia; Shimin Wu; Fengling Luo; Xiao-Lian Zhang
Journal:  Mol Ther       Date:  2014-02-27       Impact factor: 11.454

4.  Analysis of intracellular expressed proteins of Mycobacterium tuberculosis clinical isolates.

Authors:  Neelja Singhal; Prashant Sharma; Manish Kumar; Beenu Joshi; Deepa Bisht
Journal:  Proteome Sci       Date:  2012-03-01       Impact factor: 2.480

5.  Absolute quantification of selected proteins in the human osteoarthritic secretome.

Authors:  Mandy J Peffers; Robert J Beynon; Peter D Clegg
Journal:  Int J Mol Sci       Date:  2013-10-15       Impact factor: 5.923
  5 in total

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