Detection of Newcastle disease virus with quantum dots-resonance light scattering system.

A sensitive QDs-based RLS assay method for the detection of Newcastle disease virus (NDV) antibody has been developed. CdTe quantum dots (QDs) were conjugated with Newcastle disease virus and used as RLS-based probes to detect NDV antibody. The electrostatic interaction between CdTe QDs and NDV resulted in enhanced resonance light scattering (RLS) signal characterized at 555 nm. Upon the addition of NDV antibody, QDs-NDV formed dispersive immunocomplex that can decrease the RLS signal. The decreased RLS intensity at 555 nm (DeltaI(RLS)) was linearly proportional to the concentration of NDV antibody (C(anti-NDV)) in the range of 0.5-50 ng/mL, with correlation coefficient of 0.974 and detection limit of 0.1 ng/mL under the optimization conditions. The proposed method was applied to the determination of NDV antibody in spiked samples with satisfactory results. Copyright 2010 Elsevier B.V. All rights reserved.