| Literature DB >> 20685417 |
Qi Rui1, Kikuo Komori, Yang Tian, Haiqing Liu, Yongping Luo, Yasuyuki Sakai.
Abstract
In this work, direct electron transfer of cytochrome c (cyt. c)--a model for studying the electron transfer of enzymes is achieved at hexagonal ZnO nanosheets by one-step electrodeposition. UV-vis spectra and electrochemical data demonstrate that such ZnO nanosheets can supply a bio-compatible surface to keep the bioactivity of cyt. c. The redox formal potential (E(0)') of cyt. c is estimated to be 338.2+/-4.3 mV (vs. AgAgCl) at the nanostructured ZnO surface. This value is much more positive than those of enzymes previously obtained at other metal oxides and zeolite surfaces. Experiment data show, under the optimized potential of 0.0 V (vs. AgAgCl), the electrochemical determination of H(2)O(2) is free from not only anodic interferences like ascorbic acid (AA) and dopamine (DA), but also a cathodic interference-O(2). Such an excellent selectivity enable the present H(2)O(2) biosensor determine the extracellular H(2)O(2) released from living human hepatoma cells. 2010 Elsevier B.V. All rights reserved.Entities:
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Year: 2010 PMID： 20685417 DOI： 10.1016/j.aca.2010.04.065
Source DB: PubMed Journal: Anal Chim Acta ISSN： 0003-2670 Impact factor: 6.558