Literature DB >> 20684851

In vitro human TGF-beta treatment converts CD4(+)CD25(-) T cells into induced T regulatory like cells.

Revathi Shanmugasundaram1, Ramesh K Selvaraj.   

Abstract

In mammals, induced T regulatory cells (iTregs) are produced by treating CD4(+)CD25(-) cells in vitro with TGF-beta in the presence of anti-CD3 and IL-2. Chicken CD4(+)CD25(-) cells were treated in vitro with TGF-beta, anti-CD3, anti-CD28 and IL-2 and analyzed for suppressive properties, IL-10 and IL-2 mRNA amounts. TGF-beta treatment decreased (P<0.01) CD4(+)CD25(-) cell proliferation by 45% at 5d of cell culture with no increase in dead cell population. CD4(+)CD25(-) cells treated with 5ng TGF-beta for 5d had 130-fold (P<0.01) more IL-10 and 104-fold (P<0.01) less IL-2 mRNA content than the 0ng TGF-beta treated groups. CD4(+)CD25(-) cells treated with 5ng TGF-beta for 5d suppressed (P<0.01) naïve cell proliferation compared to the 0ng TGF-beta treated groups. The results suggest the presence of iTreg-like cells in avian species. Published by Elsevier B.V.

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Year:  2010        PMID： 20684851     DOI： 10.1016/j.vetimm.2010.04.017

Source DB:  PubMed          Journal:  Vet Immunol Immunopathol        ISSN： 0165-2427            Impact factor:   2.046


  7 in total

1.  Equine CD4(+) CD25(high) T cells exhibit regulatory activity by close contact and cytokine-dependent mechanisms in vitro.

Authors:  Eman Hamza; Vinzenz Gerber; Falko Steinbach; Eliane Marti
Journal:  Immunology       Date:  2011-11       Impact factor: 7.397

2.  Morphoproteomics provides support for TGF-β pathway signaling in the osteoclastogenesis and immune dysregulation of osteolytic Langerhans cell histiocytosis.

Authors:  Sanda Alexandrescu; Nina Tatevian; Bogdan A Czerniak; Michael H Covinsky; Nadja K Burns; Robert E Brown
Journal:  Int J Clin Exp Pathol       Date:  2012-07-29

3.  CD4+CD25+ Regulatory T cell ontogeny and preferential migration to the cecal tonsils in chickens.

Authors:  Revathi Shanmugasundaram; Ramesh K Selvaraj
Journal:  PLoS One       Date:  2012-03-27       Impact factor: 3.240

4.  Synbiotic supplementation to decrease Salmonella colonization in the intestine and carcass contamination in broiler birds.

Authors:  R Shanmugasundaram; M Mortada; D E Cosby; M Singh; T J Applegate; B Syed; C M Pender; S Curry; G R Murugesan; R K Selvaraj
Journal:  PLoS One       Date:  2019-10-10       Impact factor: 3.240

5.  Research Note: Effect of synbiotic supplementation on caecal Clostridium perfringens load in broiler chickens with different necrotic enteritis challenge models.

Authors:  R Shanmugasundaram; A Markazi; M Mortada; T T Ng; T J Applegate; L R Bielke; B Syed; C M Pender; S Curry; G R Murugesan; R K Selvaraj
Journal:  Poult Sci       Date:  2020-03-18       Impact factor: 3.352

6.  Missed, Not Missing: Phylogenomic Evidence for the Existence of Avian FoxP3.

Authors:  Michael P Denyer; Dammy Y Pinheiro; Oliver A Garden; Adrian J Shepherd
Journal:  PLoS One       Date:  2016-03-03       Impact factor: 3.240

7.  Prostaglandin E2 directly inhibits the conversion of inducible regulatory T cells through EP2 and EP4 receptors via antagonizing TGF-β signalling.

Authors:  Marie Goepp; Siobhan Crittenden; You Zhou; Adriano G Rossi; Shuh Narumiya; Chengcan Yao
Journal:  Immunology       Date:  2021-10-01       Impact factor: 7.397
  7 in total

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