INTRODUCTION: The cytokines that may be associated with the clearance or persistence of high-risk human papillomavirus (HPV) infection in patients with mild dysplasia or less of the uterine cervix were determined. METHODS: A prospective study of 160 patients who harbored high-risk HPV with histologically confirmed mild dysplasia or less between January 2006 and August 2008 was performed. All patients were followed up at every 4 months during the first year. Human papillomavirus DNA tests by the Hybrid Capture 2 method (Digene, Gaithersburg, Md) were performed at every visit on all patients. Blood sampling was performed in all patients at their initial visit, and an enzyme-linked immunosorbent assay was used to measure the serum levels of interferon gamma, tumor necrosis factor alpha, and interleukins 6 and 10. RESULTS: Of the 160 patients, 107 (66.9%) had clearance of HPV infection after 12 months of follow-up. The median age of patients in the persistence group was significantly higher than that of patients in the clearance group (47 vs 38 years, respectively; range, 21-77 vs 21-71 years, respectively; P = 0.0009). In univariate analysis, the number of patients with serum negative for tumor necrosis factor alpha was significantly higher in the clearance group than the persistence group (P = 0.0363). However, in multivariate logistic regression analysis, all 4 cytokines failed to show any significant association with the clearance or persistence of HPV infection. CONCLUSIONS: The baseline serum cytokine levels were not associated with the clearance or persistence of HPV infection. Systemic immunity may not influence the natural history of HPV infection.
INTRODUCTION: The cytokines that may be associated with the clearance or persistence of high-risk human papillomavirus (HPV) infection in patients with mild dysplasia or less of the uterine cervix were determined. METHODS: A prospective study of 160 patients who harbored high-risk HPV with histologically confirmed mild dysplasia or less between January 2006 and August 2008 was performed. All patients were followed up at every 4 months during the first year. Human papillomavirus DNA tests by the Hybrid Capture 2 method (Digene, Gaithersburg, Md) were performed at every visit on all patients. Blood sampling was performed in all patients at their initial visit, and an enzyme-linked immunosorbent assay was used to measure the serum levels of interferon gamma, tumor necrosis factor alpha, and interleukins 6 and 10. RESULTS: Of the 160 patients, 107 (66.9%) had clearance of HPV infection after 12 months of follow-up. The median age of patients in the persistence group was significantly higher than that of patients in the clearance group (47 vs 38 years, respectively; range, 21-77 vs 21-71 years, respectively; P = 0.0009). In univariate analysis, the number of patients with serum negative for tumor necrosis factor alpha was significantly higher in the clearance group than the persistence group (P = 0.0363). However, in multivariate logistic regression analysis, all 4 cytokines failed to show any significant association with the clearance or persistence of HPV infection. CONCLUSIONS: The baseline serum cytokine levels were not associated with the clearance or persistence of HPV infection. Systemic immunity may not influence the natural history of HPV infection.
Authors: Jennifer O Lam; Jay H Bream; Elizabeth A Sugar; Christian L Coles; Kathleen M Weber; Robert D Burk; Dorothy J Wiley; Ross D Cranston; Susheel Reddy; Joseph B Margolick; Howard D Strickler; Alicia Wentz; Lisa Jacobson; Yingshi Guo; Weihong Xiao; Maura L Gillison; Gypsyamber D'Souza Journal: Cytokine Date: 2016-04-08 Impact factor: 3.861