BACKGROUND: The aim of this study is to compare the colonization, immunoglobulin (Ig) G response, and alveolar bone loss in Aggregatibacter actinomycetemcomitans (Aa)-inoculated Fawn Hooded Hypertensive (FHH), Dahl Salt-Sensitive (DSS), and Brown Norway (BN) rats. METHODS: Each rat strain was divided into wild-type Aa-inoculated and non-inoculated control groups. Blood taken at 12 weeks after inoculation was assessed for Aa-specific IgG antibodies by an enzyme-linked immunosorbent assay. Colonization was assessed 12 weeks postinoculation. Bone loss was estimated by measuring the distance from the cemento-enamel junction (CEJ) to the alveolar bone crest (ABC) at 20 molar sites. Colonization and antibody levels were compared by using the Student t test. Diseased rats were defined as having two sites per quadrant with CEJ-ABC distances that were significantly greater than the control CEJ-ABC distances. RESULTS: The Aa colonization of FHH rats was significantly higher than in other strains (P <0.05). The Aa-specific IgG levels in the DSS Aa-inoculated group were significantly higher than in its control group (P <0.05). Only FHH rats showed Aa disease-associated bone loss (P = 0.0021). CONCLUSIONS: Aa colonized and caused more disease in FHH rats than in the other rat strains. The rat strains each responded differently to the same Aa strain.
BACKGROUND: The aim of this study is to compare the colonization, immunoglobulin (Ig) G response, and alveolar bone loss in Aggregatibacter actinomycetemcomitans (Aa)-inoculated Fawn Hooded Hypertensive (FHH), Dahl Salt-Sensitive (DSS), and Brown Norway (BN) rats. METHODS: Each rat strain was divided into wild-type Aa-inoculated and non-inoculated control groups. Blood taken at 12 weeks after inoculation was assessed for Aa-specific IgG antibodies by an enzyme-linked immunosorbent assay. Colonization was assessed 12 weeks postinoculation. Bone loss was estimated by measuring the distance from the cemento-enamel junction (CEJ) to the alveolar bone crest (ABC) at 20 molar sites. Colonization and antibody levels were compared by using the Student t test. Diseased rats were defined as having two sites per quadrant with CEJ-ABC distances that were significantly greater than the control CEJ-ABC distances. RESULTS: The Aa colonization of FHHrats was significantly higher than in other strains (P <0.05). The Aa-specific IgG levels in the DSSAa-inoculated group were significantly higher than in its control group (P <0.05). Only FHHrats showed Aa disease-associated bone loss (P = 0.0021). CONCLUSIONS:Aa colonized and caused more disease in FHHrats than in the other rat strains. The rat strains each responded differently to the same Aa strain.
Authors: Beatriz de Brito Bezerra; Oelisoa Andriankaja; Jun Kang; Sandra Pacios; Hyung Jin Bae; Yu Li; Vincent Tsiagbe; Helen Schreiner; Daniel H Fine; Dana T Graves Journal: J Clin Periodontol Date: 2012-02-07 Impact factor: 8.728
Authors: Antje Bast; Helen Kubis; Birte Holtfreter; Silvia Ribback; Heiner Martin; Helen C Schreiner; Malte J Dominik; Katrin Breitbach; Frank Dombrowski; Thomas Kocher; Ivo Steinmetz Journal: Infect Immun Date: 2017-01-26 Impact factor: 3.441
Authors: Jun Kang; Beatriz de Brito Bezerra; Sandra Pacios; Oelisoa Andriankaja; Yu Li; Vincent Tsiagbe; Helen Schreiner; Daniel H Fine; Dana T Graves Journal: Infect Immun Date: 2012-03-26 Impact factor: 3.441
Authors: S K Velusamy; R Poojary; R Ardeshna; Waad Alabdulmohsen; D H Fine; K Velliyagounder Journal: Antimicrob Agents Chemother Date: 2013-11-04 Impact factor: 5.191