Literature DB >> 20678519

Composition analysis and anti-hypoxia activity of polysaccharide from Brassica rapa L.

Yue Xie1, Siping Jiang, Donghai Su, Ningning Pi, Chao Ma, Ping Gao.   

Abstract

Crude water-soluble polysaccharides (BRP) were extracted from the root of Brassica rapa L. using boiling-water. The polysaccharides were successively purified by chromatography on DEAE-cellulose and Sephadex G-100 column, giving three major polysaccharide fractions termed BRP1-1, BRP2-1, BRP3-1. The gel permeation chromatography (GPC) analysis showed that the average molecular weight (Mw) of polysaccharides (BRP1-1, BRP2-1, BRP3-1) were approximately 5.53×10(3) Da, 3.35×10(4) Da and 3.37×10(4) Da, respectively. Monosaccharide components analysis indicated that BRP1-1 was composed of arabinose and glucose in a molar ratio of 1.66:98.34. BRP2-1 was composed of arabinose, galactose and glucose in a molar ratio of 9.3:14.63:76.07. BRP3-1 was composed of arabinose, rhamnose, galactose and glucose in a molar ratio of 24.98:24.10:44.09:6.83. The evaluation of anti-hypoxia activity in vivo revealed that BRP is a novel potential anti-hypoxia agent.
Copyright © 2010 Elsevier B.V. All rights reserved.

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Year:  2010        PMID： 20678519     DOI： 10.1016/j.ijbiomac.2010.07.008

Source DB:  PubMed          Journal:  Int J Biol Macromol        ISSN： 0141-8130            Impact factor:   6.953


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