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Literature DB >> 20675450

Restoration of growth phenotypes of Escherichia coli DH5alpha in minimal media through reversal of a point mutation in purB.

Suk-Chae Jung¹, Chris L Smith, Ki-Sung Lee, Min-Eui Hong, Dae-Hyuk Kweon, Gregory Stephanopoulos, Yong-Su Jin.

Abstract

A point mutation (E115K) resulting in slower growth of Escherichia coli DH5alpha and XL1-Blue in minimal media was identified in the purB gene, coding for adenylosuccinate lyase (ASL), through complementation with an E. coli K-12 genomic library and serial subcultures. Chromosomal modification reversing the mutation to the wild type restored growth phenotypes in minimal media.

Entities: Mutation Species

Mesh：

Substances：

Year: 2010 PMID： 20675450 PMCID： PMC2937491 DOI： 10.1128/AEM.01210-10

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

11 in total

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Authors: K A Datsenko; B L Wanner
Journal: Proc Natl Acad Sci U S A Date: 2000-06-06 Impact factor: 11.205

6. Characterization of lycopene-overproducing E. coli strains in high cell density fermentations.

Authors: Hal Alper; Kohei Miyaoku; Gregory Stephanopoulos
Journal: Appl Microbiol Biotechnol Date: 2006-02-25 Impact factor: 4.813

7. Improvement of xylose uptake and ethanol production in recombinant Saccharomyces cerevisiae through an inverse metabolic engineering approach.

Authors: Yong-Su Jin; Hal Alper; Yea-Tyng Yang; Gregory Stephanopoulos
Journal: Appl Environ Microbiol Date: 2005-12 Impact factor: 4.792

8. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements.

Authors: R Lutz; H Bujard
Journal: Nucleic Acids Res Date: 1997-03-15 Impact factor: 16.971

6. Spectinomycin resistance in Lysobacter enzymogenes is due to its rRNA target but also relies on cell-wall recycling and purine biosynthesis.

Authors: Menghao Yu; Youfu Zhao
Journal: Front Microbiol Date: 2022-08-31 Impact factor: 6.064

7. Complete Genome Sequence of Escherichia coli ER1821R, a Laboratory K-12 Derivative Engineered To Be Deficient in All Methylcytosine and Methyladenine Restriction Systems.

Authors: Michael G Jobling; Elisabeth A Raleigh; Daniel N Frank
Journal: Genome Announc Date: 2016-08-11

7 in total

Restoration of growth phenotypes of Escherichia coli DH5alpha in minimal media through reversal of a point mutation in purB.

Review 1. High-cell-density cultivation of Escherichia coli.

Review 2. Toward a science of metabolic engineering.

3. Identifying gene targets for the metabolic engineering of lycopene biosynthesis in Escherichia coli.

Review 4. Plasmid transformation of Escherichia coli and other bacteria.

5. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.

6. Characterization of lycopene-overproducing E. coli strains in high cell density fermentations.

7. Improvement of xylose uptake and ethanol production in recombinant Saccharomyces cerevisiae through an inverse metabolic engineering approach.

8. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements.

9. High cell density fermentation of recombinant Escherichia coli expressing human interferon alpha 1.

10. Multi-dimensional gene target search for improving lycopene biosynthesis in Escherichia coli.

1. Bioproduction of Linalool From Paper Mill Waste.

2. Multi-omics Quantification of Species Variation of Escherichia coli Links Molecular Features with Strain Phenotypes.

3. In vivo kinetics of segregation and polar retention of MS2-GFP-RNA complexes in Escherichia coli.

4. In vitro evolution of an archetypal enteropathogenic Escherichia coli strain.

5. Efficient Production of Single-Stranded Phage DNA as Scaffolds for DNA Origami.

6. Spectinomycin resistance in Lysobacter enzymogenes is due to its rRNA target but also relies on cell-wall recycling and purine biosynthesis.

7. Complete Genome Sequence of Escherichia coli ER1821R, a Laboratory K-12 Derivative Engineered To Be Deficient in All Methylcytosine and Methyladenine Restriction Systems.