| Literature DB >> 20674370 |
Chenguang Lou1, Qiang Xiao, Lavinia Brennan, Mark E Light, Nuria Vergara-Irigaray, Elizabeth M Atkinson, Lindy M Holden-Dye, Keith R Fox, Tom Brown.
Abstract
2'-O-(2-Methoxyethyl)-5-(3-aminoprop-1-ynyl)-uridine phosphoramidite (MEPU) has been synthesized from d-ribose and 5-iodouracil and incorporated into triplex-forming oligonucleotides (TFOs) by automated solid-phase oligonucleotide synthesis. The TFOs gave very high triplex stability with their target duplexes as measured by ultraviolet/fluorescence melting and DNase I footprinting. The incorporation of MEPU into TFOs renders them resistant to degradation by serum nucleases. Copyright 2010 Elsevier Ltd. All rights reserved.Entities:
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Year: 2010 PMID： 20674370 DOI： 10.1016/j.bmc.2010.07.005
Source DB: PubMed Journal: Bioorg Med Chem ISSN： 0968-0896 Impact factor: 3.641