| Literature DB >> 20673938 |
Megumi Sato1, Surapol Sanguankiat, Tippayarat Yoonuan, Tiengkham Pongvongsa, Malaythong Keomoungkhoun, Inthava Phimmayoi, Boungnong Boupa, Kazuhiko Moji, Jitra Waikagul.
Abstract
The prevalence of hookworm eggs in fecal samples, by modified cellophane thick-smear technique, was found to be 30.0% (61/203 samples) in Lahanam Village, Savannakhet Province, Lao PDR. The hookworm eggs were morphologically heterogeneous, so that identification was confirmed by copro-PCR with specific primers for hookworms and Trichostrongylus spp.; 12 samples were positive for Necator americanus, 19 for Ancylostoma spp., with one mixed infection of both. Sequencing of the Ancylostoma spp. copro-PCR products found A. duodenale, and also the animal hookworms, A. caninum and A. ceylanicum. Moreover, Trichostrongylus spp.-specific copro-PCR revealed a significant rate of infection (43/203; 21.2%). Sequencing confirmed the zoonotic species, T. colubriformis. PCR/sequencing is useful for differentiating parasite species. The positives by the Kato-Katz method, and by the combined hookworm/Trichostrongylus specific-PCR, were comparable, although the agreement between the two methodologies was only 50%. Copyright 2010 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.Entities:
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Year: 2010 PMID： 20673938 DOI： 10.1016/j.trstmh.2010.06.006
Source DB: PubMed Journal: Trans R Soc Trop Med Hyg ISSN： 0035-9203 Impact factor: 2.184