| Literature DB >> 20673212 |
L E Petrovskaya1, A A Shulga, O V Bocharova, Ya S Ermolyuk, E A Kryukova, V V Chupin, M J J Blommers, A S Arseniev, M P Kirpichnikov.
Abstract
To elaborate a high-performance system for expression of genes of G-protein coupled receptors (GPCR), methods of direct and hybrid expression of 17 GPCR genes in Escherichia coli and selection of strains and bacteria cultivation conditions were investigated. It was established that expression of most of the target GPCR fused with the N-terminal fragment of OmpF or Mistic using media for autoinduction provides high output (up to 50 mg/liter).Entities:
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Year: 2010 PMID： 20673212 DOI： 10.1134/s0006297910070102
Source DB: PubMed Journal: Biochemistry (Mosc) ISSN： 0006-2979 Impact factor: 2.487