Enhanced bactericidal activity of modified titania in sunlight against Pseudomonas aeruginosa, a water-borne pathogen.

Photocatalyst-mediated inactivations generate reactive oxygen species and OH radicals, which induce oxidative destruction of membrane integrity, causing damage to membrane phospholipids of gram negative bacteria like Pseudomonas aeruginosa. Nanosized TiO(2) was synthesized by gel to crystalline conversion and Zr-doped TiO(2) was synthesized by pulverization using appropriate precursor. The doped nanocrystals retained the anatase phase with a marginal increase in crystallite size, averaging at 25nm. SEM-EDX analysis of the doped sample depicts the substantial growth of grain size with 1.33 atomic weight % of zirconium. The created electron states in the doped sample act as charge carrier traps suppressing recombination which later detraps the same to the surface of the catalyst causing enhanced interfacial charge transfer. Zr-doped TiO(2) at the molecular scale exhibits better photocatalytic activity with lower bandgap energy that can respond to visible light. The redshift caused by the dopants in absorption spectra of TiO(2) facilitated the nonintrinsic sample to exhibit nearly 2-fold enhancement of photoinactivation in sunlight. Extent of photoinactivation of P. aeruginosa was observed to be complete (100%) within 150min of sunlight exposure in the presence of modified TiO(2) .