BACKGROUND: Molecular diagnostic tests to detect enterovirus in clinical specimens usually target highly conserved sites in the 5'-non-translated region, allowing detection of all members of the genus. The sequences in the 5'-NTR do not correlate with serotype, but PCR and sequencing of the VP1 region can be used for typing; this system has largely replaced traditional antigenic typing. OBJECTIVE: To investigate the relative performance of two common enterovirus assays. STUDY DESIGN: We compared the relative sensitivities of Taqman real-time RT-PCR (rRT-PCR) and a VP1 semi-nested PCR (RT-snPCR) assay in which sequencing the VP1 amplicon also provides typing information. RESULTS: There was 89% concordance between the two methods among the 371 clinical specimens tested (74 positive in both assays and 257 negative in both assays). Twenty-seven rRT-PCR-negative/VP1-positive specimens were confirmed positive by sequencing; 13 specimens were rRT-PCR-positive/VP1-negative. CONCLUSIONS: The results suggest that either assay can produce satisfactory results, but that using both assays in parallel should provide the highest sensitivity for clinical diagnostic testing. Published by Elsevier B.V.
BACKGROUND: Molecular diagnostic tests to detect enterovirus in clinical specimens usually target highly conserved sites in the 5'-non-translated region, allowing detection of all members of the genus. The sequences in the 5'-NTR do not correlate with serotype, but PCR and sequencing of the VP1 region can be used for typing; this system has largely replaced traditional antigenic typing. OBJECTIVE: To investigate the relative performance of two common enterovirus assays. STUDY DESIGN: We compared the relative sensitivities of Taqman real-time RT-PCR (rRT-PCR) and a VP1 semi-nested PCR (RT-snPCR) assay in which sequencing the VP1 amplicon also provides typing information. RESULTS: There was 89% concordance between the two methods among the 371 clinical specimens tested (74 positive in both assays and 257 negative in both assays). Twenty-seven rRT-PCR-negative/VP1-positive specimens were confirmed positive by sequencing; 13 specimens were rRT-PCR-positive/VP1-negative. CONCLUSIONS: The results suggest that either assay can produce satisfactory results, but that using both assays in parallel should provide the highest sensitivity for clinical diagnostic testing. Published by Elsevier B.V.
Authors: Todd F Hatchette; Steven J Drews; Elsie Grudeski; Tim Booth; Christine Martineau; Kerry Dust; Richard Garceau; Jonathan Gubbay; Tim Karnauchow; Mel Krajden; Paul N Levett; Tony Mazzulli; Ryan R McDonald; Alan McNabb; Samira Mubareka; Robert Needle; Astrid Petrich; Susan Richardson; Candy Rutherford; Marek Smieja; Raymond Tellier; Graham Tipples; Jason J LeBlanc Journal: J Clin Microbiol Date: 2015-03-04 Impact factor: 5.948
Authors: Julie A Boom; Leila C Sahni; Daniel C Payne; Rashi Gautam; Freda Lyde; Slavica Mijatovic-Rustempasic; Michael D Bowen; Jacqueline E Tate; Marcia A Rench; Jon R Gentsch; Umesh D Parashar; Carol J Baker Journal: J Infect Dis Date: 2012-08-07 Impact factor: 5.226
Authors: Olga D Lorenzi; Christopher J Gregory; Luis Manuel Santiago; Héctor Acosta; Ivonne E Galarza; Elizabeth Hunsperger; Jorge Muñoz; Duy M Bui; M Steven Oberste; Silvia Peñaranda; Carlos García-Gubern; Kay M Tomashek Journal: Am J Trop Med Hyg Date: 2013-02-04 Impact factor: 2.345
Authors: M Steven Oberste; Mohammed M Feeroz; Kaija Maher; W Allan Nix; Gregory A Engel; Kamrul M Hasan; Sajeda Begum; Gunwha Oh; Anwarul H Chowdhury; Mark A Pallansch; Lisa Jones-Engel Journal: J Virol Date: 2012-10-24 Impact factor: 5.103
Authors: Andrés Lizasoain; Fernanda M Burlandy; Matías Victoria; Luis F López Tort; Edson E da Silva; Rodney Colina Journal: Food Environ Virol Date: 2018-06-16 Impact factor: 2.778
Authors: Dena R Shibib; Scott M Matushek; Kathleen G Beavis; Susan H Gawel; Angella Charnot-Katsikas Journal: J Clin Microbiol Date: 2015-11-25 Impact factor: 5.948