OBJECTIVE: To test the ability of Bio-Oss in inducing growth factors and proinflammatory cytokines that may have a role in inflammation after grafting, bone resorption, remodeling and in the homeostasis of osteoblasts. MATERIAL AND METHODS: Normal human osteoblasts were seeded in Petri dishes containing granules of Bio-Oss, cells were harvested after confluency and RNA was extracted. Reverse transcriptase polymerase chain reaction was performed using specific primers for osteonectin, bone sialoprotein (BSP), bone morphogenetic protein (BMP)-2 and BMP-7, platelet-derived growth factor (PDGF), interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-alpha) and integrin beta1. Glycerol-3-phosphate dehydrogenase was used as the housekeeping gene and normal human osteoblasts grown on Petri dishes without Bio-Oss granules were used as negative controls. RESULTS: Osteoblast grown on Bio-Oss showed a normal RNA expression of osteonectin, integrin beta1 and PDGF. However, compared with control osteoblasts it showed a reduced expression of BSP, BMP-2 and BMP-7, IL-6 and TNF-alpha. CONCLUSIONS: Our findings further support the evidence that Bio-Oss is an excellent biomaterial that does not enhance the production of proinflammatory cytokines.
OBJECTIVE: To test the ability of Bio-Oss in inducing growth factors and proinflammatory cytokines that may have a role in inflammation after grafting, bone resorption, remodeling and in the homeostasis of osteoblasts. MATERIAL AND METHODS: Normal human osteoblasts were seeded in Petri dishes containing granules of Bio-Oss, cells were harvested after confluency and RNA was extracted. Reverse transcriptase polymerase chain reaction was performed using specific primers for osteonectin, bone sialoprotein (BSP), bone morphogenetic protein (BMP)-2 and BMP-7, platelet-derived growth factor (PDGF), interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-alpha) and integrin beta1. Glycerol-3-phosphate dehydrogenase was used as the housekeeping gene and normal human osteoblasts grown on Petri dishes without Bio-Oss granules were used as negative controls. RESULTS: Osteoblast grown on Bio-Oss showed a normal RNA expression of osteonectin, integrin beta1 and PDGF. However, compared with control osteoblasts it showed a reduced expression of BSP, BMP-2 and BMP-7, IL-6 and TNF-alpha. CONCLUSIONS: Our findings further support the evidence that Bio-Oss is an excellent biomaterial that does not enhance the production of proinflammatory cytokines.
Authors: Margherita Tumedei; Eitan Mijiritsky; Carlos Fernando Mourão; Adriano Piattelli; Marco Degidi; Carlo Mangano; Giovanna Iezzi Journal: Int J Environ Res Public Health Date: 2022-06-28 Impact factor: 4.614
Authors: Mércia J S Cunha; Luis A Esper; Michyele C Sbrana; Paula G F P de Oliveira; Accácio L do Valle; Ana Lúcia P F de Almeida Journal: Biomed Res Int Date: 2014-05-28 Impact factor: 3.411
Authors: Pedro Henrique de Azambuja Carvalho; Sarah Al-Maawi; Eva Dohle; Robert Alexander Sader; Valfrido Antonio Pereira-Filho; Shahram Ghanaati Journal: Materials (Basel) Date: 2022-01-27 Impact factor: 3.623