Literature DB >> 2066346

Use of an Escherichia coli beta-glucuronidase gene as a reporter gene for investigation of Bacteroides promoters.

M J Feldhaus1, V Hwa, Q Cheng, A A Salyers.   

Abstract

We have constructed transcriptional fusion vectors for use in Bacteroides spp., a genus of gram-negative obligate anaerobes found in high numbers in the human colon. The reporter group in these vectors is a promoterless beta-glucuronidase gene from Escherichia coli (uidA). Two of the vectors (pMJF-2 and pMJF-3) replicate in Bacteroides spp. The third, pCQW-1, does not replicate in Bacteroides spp. and can be used to introduce E. coli beta-glucuroindase fusions into the Bacteroides chromosome.

Entities:  

Mesh：

Substances：

Year:  1991        PMID： 2066346      PMCID： PMC208121          DOI： 10.1128/jb.173.14.4540-4543.1991

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  9 in total

1.  Nucleotide sequence analysis of Tn4551: use of ermFS operon fusions to detect promoter activity in Bacteroides fragilis.

Authors:  C J Smith
Journal:  J Bacteriol       Date:  1987-10       Impact factor: 3.490

2.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

3.  Mobilization of Bacteroides plasmids by Bacteroides conjugal elements.

Authors:  P J Valentine; N B Shoemaker; A A Salyers
Journal:  J Bacteriol       Date:  1988-03       Impact factor: 3.490

4.  Complete nucleotide sequence and transcription of ermF, a macrolide-lincosamide-streptogramin B resistance determinant from Bacteroides fragilis.

Authors:  J L Rasmussen; D A Odelson; F L Macrina
Journal:  J Bacteriol       Date:  1986-11       Impact factor: 3.490

5.  Use of targeted insertional mutagenesis to determine whether chondroitin lyase II is essential for chondroitin sulfate utilization by Bacteroides thetaiotaomicron.

Authors:  E P Guthrie; A A Salyers
Journal:  J Bacteriol       Date:  1986-06       Impact factor: 3.490

6.  A cryptic 65-kilobase-pair transposonlike element isolated from Bacteroides uniformis has homology with Bacteroides conjugal tetracycline resistance elements.

Authors:  N B Shoemaker; A A Salyers
Journal:  J Bacteriol       Date:  1990-04       Impact factor: 3.490

7.  beta-Glucuronidase from Escherichia coli as a gene-fusion marker.

Authors:  R A Jefferson; S M Burgess; D Hirsh
Journal:  Proc Natl Acad Sci U S A       Date:  1986-11       Impact factor: 11.205

8.  Development and use of cloning systems for Bacteroides fragilis: cloning of a plasmid-encoded clindamycin resistance determinant.

Authors:  C J Smith
Journal:  J Bacteriol       Date:  1985-10       Impact factor: 3.490

9.  Location and nucleotide sequence of the transfer origin of the broad host range plasmid RK2.

Authors:  D G Guiney; E Yakobson
Journal:  Proc Natl Acad Sci U S A       Date:  1983-06       Impact factor: 11.205
  9 in total
  31 in total

1.  Integration and excision of a Bacteroides conjugative transposon, CTnDOT.

Authors:  Q Cheng; B J Paszkiet; N B Shoemaker; J F Gardner; A A Salyers
Journal:  J Bacteriol       Date:  2000-07       Impact factor: 3.490

2.  Production of two proteins encoded by the Bacteroides mobilizable transposon NBU1 correlates with time-dependent accumulation of the excised NBu1 circular form.

Authors:  J Wang; G R Wang; N B Shoemaker; A A Salyers
Journal:  J Bacteriol       Date:  2001-11       Impact factor: 3.490

3.  Characterization of the Bacteroides CTnDOT regulatory protein RteC.

Authors:  Jiyeon Park; Abigail A Salyers
Journal:  J Bacteriol       Date:  2010-10-29       Impact factor: 3.490

4.  Genes involved in production of plasmidlike forms by a Bacteroides conjugal chromosomal element share amino acid homology with two-component regulatory systems.

Authors:  A M Stevens; J M Sanders; N B Shoemaker; A A Salyers
Journal:  J Bacteriol       Date:  1992-05       Impact factor: 3.490

5.  Cloning and partial characterization of two chromosomal loci from Bacteroides ovatus that contain genes essential for growth on guar gum.

Authors:  P J Valentine; P Arnold; A A Salyers
Journal:  Appl Environ Microbiol       Date:  1992-05       Impact factor: 4.792

6.  Translational control of tetracycline resistance and conjugation in the Bacteroides conjugative transposon CTnDOT.

Authors:  Yanping Wang; Ella R Rotman; Nadja B Shoemaker; Abigail A Salyers
Journal:  J Bacteriol       Date:  2005-04       Impact factor: 3.490

7.  Regulation of excision genes of the Bacteroides conjugative transposon CTnDOT.

Authors:  Kyung Moon; Nadja B Shoemaker; Jeffrey F Gardner; Abigail A Salyers
Journal:  J Bacteriol       Date:  2005-08       Impact factor: 3.490

8.  The Bacteroides mobilizable insertion element, NBU1, integrates into the 3' end of a Leu-tRNA gene and has an integrase that is a member of the lambda integrase family.

Authors:  N B Shoemaker; G R Wang; A A Salyers
Journal:  J Bacteriol       Date:  1996-06       Impact factor: 3.490

9.  Activities of the Porphyromonas gingivalis PrtP proteinase determined by construction of prtP-deficient mutants and expression of the gene in Bacteroides species.

Authors:  G A Barkocy-Gallagher; J W Foley; M S Lantz
Journal:  J Bacteriol       Date:  1999-01       Impact factor: 3.490

10.  Tetracycline regulation of genes on Bacteroides conjugative transposons.

Authors:  A M Stevens; N B Shoemaker; L Y Li; A A Salyers
Journal:  J Bacteriol       Date:  1993-10       Impact factor: 3.490
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.