OBJECTIVES: The aim was to determine the chemical composition of the essential oil of Kadsura longipedunculata and the biological activity of the oil and its major components. METHODS: The essential oil from stem bark of Kadsura longipedunculata was analysed by capillary gas chromatography (GLC/FID) and gas chromatography-mass spectrometry (GLC/MS). The ability of the oil to reduce diphenylpicrylhydrazine (DPPH(*)) was used to evaluate the antioxidant activity. Inhibition of both lipoxygenase and prostaglandin E(2) was used to assess the anti-inflammatory activity. Antimicrobial activity was studied in vitro against a range of bacteria and fungi using diffusion and microdilution methods. Inhibition of trypanosome proliferation was assessed using resazurin as vital stain. The in-vitro cytotoxicity of the essential oil on six human cancer cell lines (HepG2, MIA PaCa-2, HeLa, HL-60, MDA-MB-231 and SW-480) was examined using the MTT assay. KEY FINDINGS: Fifty compounds, representing 97.63% of total oil, were identified. delta-Cadinene (21.79%), camphene (7.27%), borneol (6.05%), cubenol (5.12%) and delta-cadinol (5.11%) were found to be the major components of the oil. The oil exerted a good antimicrobial activity against all Gram-positive bacteria tested, including methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus faecalis. Streptococcus pyogenes and S. agalactiae were the most sensitive bacteria with a minimal inhibitory concentration (MIC) of 60 microg/ml oil. The essential oil showed a moderate fungicidal activity against yeasts, but it did not show any activity against Gram-negative bacteria. The essential oil showed a good trypanocidal activity in Trypanosoma b. brucei with an IC50 value of 50.52 +/- 0.029 microg/ml. Radical scavenging activity had an IC50 value of 3.06 +/- 0.79 mg/ml. 5-Lipoxygenase inhibition (IC50 = 38.58 microg/ml) and prostaglandin E(2) production inhibition (28.82% at 25 microg/ml) accounted for anti-inflammatory activity of the oil. The oil exhibited some degree of cytotoxic activity against MIA PaCa-2, HepG-2 and SW-480 cell lines with IC50 values of 133.53, 136.96 and 136.62 microg/ml, respectively. The oil increased caspase 3/7 activity (an indicator of apoptosis) 2.5-4 fold in MIA Paca-2 cells. Camphene and borneol did not show antioxidant activity. However, both compounds exhibited some degree of antimicrobial, trypanocidal, anti-inflammatory and cytotoxic activity. CONCLUSIONS: This investigation provided evidence for, and confirmed the efficacy of, K. longipedunculata, a traditionally used Chinese medicinal plant for the treatment of inflammation and infection.
OBJECTIVES: The aim was to determine the chemical composition of the essential oil of Kadsura longipedunculata and the biological activity of the oil and its major components. METHODS: The essential oil from stem bark of Kadsura longipedunculata was analysed by capillary gas chromatography (GLC/FID) and gas chromatography-mass spectrometry (GLC/MS). The ability of the oil to reduce diphenylpicrylhydrazine (DPPH(*)) was used to evaluate the antioxidant activity. Inhibition of both lipoxygenase and prostaglandin E(2) was used to assess the anti-inflammatory activity. Antimicrobial activity was studied in vitro against a range of bacteria and fungi using diffusion and microdilution methods. Inhibition of trypanosome proliferation was assessed using resazurin as vital stain. The in-vitro cytotoxicity of the essential oil on six humancancer cell lines (HepG2, MIA PaCa-2, HeLa, HL-60, MDA-MB-231 and SW-480) was examined using the MTT assay. KEY FINDINGS: Fifty compounds, representing 97.63% of total oil, were identified. delta-Cadinene (21.79%), camphene (7.27%), borneol (6.05%), cubenol (5.12%) and delta-cadinol (5.11%) were found to be the major components of the oil. The oil exerted a good antimicrobial activity against all Gram-positive bacteria tested, including methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus faecalis. Streptococcus pyogenes and S. agalactiae were the most sensitive bacteria with a minimal inhibitory concentration (MIC) of 60 microg/ml oil. The essential oil showed a moderate fungicidal activity against yeasts, but it did not show any activity against Gram-negative bacteria. The essential oil showed a good trypanocidal activity in Trypanosoma b. brucei with an IC50 value of 50.52 +/- 0.029 microg/ml. Radical scavenging activity had an IC50 value of 3.06 +/- 0.79 mg/ml. 5-Lipoxygenase inhibition (IC50 = 38.58 microg/ml) and prostaglandin E(2) production inhibition (28.82% at 25 microg/ml) accounted for anti-inflammatory activity of the oil. The oil exhibited some degree of cytotoxic activity against MIA PaCa-2, HepG-2 and SW-480 cell lines with IC50 values of 133.53, 136.96 and 136.62 microg/ml, respectively. The oil increased caspase 3/7 activity (an indicator of apoptosis) 2.5-4 fold in MIA Paca-2 cells. Camphene and borneol did not show antioxidant activity. However, both compounds exhibited some degree of antimicrobial, trypanocidal, anti-inflammatory and cytotoxic activity. CONCLUSIONS: This investigation provided evidence for, and confirmed the efficacy of, K. longipedunculata, a traditionally used Chinese medicinal plant for the treatment of inflammation and infection.
Authors: Mahmoud Youns; Momen Askoura; Hisham A Abbas; Gouda H Attia; Ahdab N Khayyat; Reham M Goda; Ahmad J Almalki; El-Sayed Khafagy; Wael A H Hegazy Journal: Onco Targets Ther Date: 2021-06-23 Impact factor: 4.147
Authors: Deivid C Soares; Nathalya A Portella; Mônica Freiman de S Ramos; Antonio C Siani; Elvira M Saraiva Journal: Evid Based Complement Alternat Med Date: 2013-06-22 Impact factor: 2.629