Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 The length polymorphism in the 5' flanking region of the human beta-globin gene with denaturing gradient gel electrophoresis in a Japanese population.

Literature DB >> 2066111

The length polymorphism in the 5' flanking region of the human beta-globin gene with denaturing gradient gel electrophoresis in a Japanese population.

N Takahashi¹, K Hiyama, M Kodaira, C Satoh.

Abstract

The ATTTT repeat polymorphism located approximately 1,400 base pairs (bp) upstream from the beta-globin structural gene was analyzed by denaturing gradient gel electrophoresis (DGGE) of RNA:DNA duplexes. A study of 81 unrelated Japanese from Hiroshima revealed a sequence heteromorphism in this site. The alleles with five and six repeats of the ATTTT unit, which have been reported, were found in polymorphic proportions. Two unreported alleles were also detected, the first, in two persons, characterized by seven repeats and the other, in a single person, having an A-to-G nucleotide substitution in the fifth repeat.

Entities: Species

Mesh：

Substances：
Globins

Year: 1991 PMID： 2066111 DOI： 10.1007/BF00204187

Source DB: PubMed Journal: Hum Genet ISSN： 0340-6717 Impact factor: 4.132

7 in total

1. Detection of deletions, insertions and single nucleotide substitutions in cloned beta-globin genes and new polymorphic nucleotide substitutions in beta-globin genes in a Japanese population using ribonuclease cleavage at mismatches in RNA:DNA duplexes.

Authors: K Hiyama; M Kodaira; C Satoh
Journal: Mutat Res Date: 1990-08 Impact factor: 2.433

1 in total

1. A pentanucleotide repeat polymorphism in the 5' control region of the apolipoprotein(a) gene is associated with lipoprotein(a) plasma concentrations in Caucasians.

Authors: M Trommsdorff; S Köchl; A Lingenhel; F Kronenberg; R Delport; H Vermaak; L Lemming; I C Klausen; O Faergeman; G Utermann
Journal: J Clin Invest Date: 1995-07 Impact factor: 14.808

1 in total

The length polymorphism in the 5' flanking region of the human beta-globin gene with denaturing gradient gel electrophoresis in a Japanese population.

1. Detection of deletions, insertions and single nucleotide substitutions in cloned beta-globin genes and new polymorphic nucleotide substitutions in beta-globin genes in a Japanese population using ribonuclease cleavage at mismatches in RNA:DNA duplexes.

2. An improved method for the detection of genetic variations in DNA with denaturing gradient gel electrophoresis.

3. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

4. Detection and localization of single base changes by denaturing gradient gel electrophoresis.

5. Dideoxy sequencing method using denatured plasmid templates.

6. Duplication/deletion polymorphism 5' - to the human beta globin gene.

7. Characterization of beta-thalassaemia mutations using direct genomic sequencing of amplified single copy DNA.

1. A pentanucleotide repeat polymorphism in the 5' control region of the apolipoprotein(a) gene is associated with lipoprotein(a) plasma concentrations in Caucasians.