| Literature DB >> 20659687 |
Fedor V Subach1, Lijuan Zhang, Theodorus W J Gadella, Nadya G Gurskaya, Konstantin A Lukyanov, Vladislav V Verkhusha.
Abstract
We have developed the first red fluorescent protein, named rsTagRFP, which possesses reversibly photoswitchable absorbance spectra. Illumination with blue and yellow light switches rsTagRFP into a red fluorescent state (ON state) or nonfluorescent state (OFF state), respectively. The ON and OFF states exhibit absorbance maxima at 567 and 440 nm, respectively. Due to the photoswitchable absorbance, rsTagRFP can be used as an acceptor for a photochromic Förster resonance energy transfer (pcFRET). The photochromic acceptor facilitates determination of a protein-protein interaction by providing an internal control for FRET. Using pcFRET with EYFP as a donor, we observed an interaction between epidermal growth factor receptor and growth factor receptor-binding protein 2 in live cells by detecting the modulation of both the fluorescence intensity and lifetime of the EYFP donor upon the ON-OFF photoswitching of the rsTagRFP acceptor. 2010 Elsevier Ltd. All rights reserved.Entities:
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Year: 2010 PMID： 20659687 PMCID： PMC2911641 DOI： 10.1016/j.chembiol.2010.05.022
Source DB: PubMed Journal: Chem Biol ISSN： 1074-5521