OBJECTIVE: To assess the clinical value of bisulfite polymerase chain reaction Luminex (BPL), an automated, high-throughput procedure for the detection of alterations in DNA methylation. DESIGN: Experimental prospective study. SETTING: University research laboratory and private in vitro fertilization (IVF) clinic. PATIENT(S): A total of 337 men, 61 with severe oligozoospermia, 67 with moderate oligozoospermia, and 209 with microscopically normozoospermia. INTERVENTION(S): The ejaculated sperm samples after the routine semen analysis with patients' consent. MAIN OUTCOME MEASURE(S): Examination of the methylation patterns of eight imprinted loci in sperm DNA, and confirmation with combined bisulfite PCR restriction analysis (COBRA). RESULT(S): A total of 47 cases (13.9%) showed abnormal methylation at one or more imprinted loci (18 paternal, 18 maternal, and 11 cases with alterations of both maternal and paternal imprints). CONCLUSION(S): The relative ease of the BPL method provides a practical method within a clinical setting to reduce the likelihood of abnormal samples being used in assisted reproduction treatments.
OBJECTIVE: To assess the clinical value of bisulfite polymerase chain reaction Luminex (BPL), an automated, high-throughput procedure for the detection of alterations in DNA methylation. DESIGN: Experimental prospective study. SETTING: University research laboratory and private in vitro fertilization (IVF) clinic. PATIENT(S): A total of 337 men, 61 with severe oligozoospermia, 67 with moderate oligozoospermia, and 209 with microscopically normozoospermia. INTERVENTION(S): The ejaculated sperm samples after the routine semen analysis with patients' consent. MAIN OUTCOME MEASURE(S): Examination of the methylation patterns of eight imprinted loci in sperm DNA, and confirmation with combined bisulfite PCR restriction analysis (COBRA). RESULT(S): A total of 47 cases (13.9%) showed abnormal methylation at one or more imprinted loci (18 paternal, 18 maternal, and 11 cases with alterations of both maternal and paternal imprints). CONCLUSION(S): The relative ease of the BPL method provides a practical method within a clinical setting to reduce the likelihood of abnormal samples being used in assisted reproduction treatments.
Authors: Victoria K Cortessis; Kimberly Siegmund; Sahar Houshdaran; Peter W Laird; Rebecca Z Sokol Journal: Fertil Steril Date: 2011-10-26 Impact factor: 7.329
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Authors: Sara E Pacheco; E Andres Houseman; Brock C Christensen; Carmen J Marsit; Karl T Kelsey; Mark Sigman; Kim Boekelheide Journal: PLoS One Date: 2011-06-02 Impact factor: 3.240
Authors: A P A van Montfoort; L L P Hanssen; P de Sutter; S Viville; J P M Geraedts; P de Boer Journal: Hum Reprod Update Date: 2012-01-19 Impact factor: 15.610