Electrochemical behavior and analytical application of ciprofloxacin using a multi-walled nanotube composite film-glassy carbon electrode.

A simple, rapid and applicable electrochemical method was developed for the determination of ciprofloxacin (Cf) based on a multi-wall carbon nanotubes film-modified glassy carbon electrode (MWCNT/GCE). The constructed electrode (MWCNT/GCE) exhibited excellent electrocatalytic behavior in the oxidation of Cf as evidenced by the enhancement of the oxidation peak current and the shift in the oxidation potential to lower values (by 130 mV) in comparison with the bare GCE. A detailed analysis of cyclic voltammograms and chronoamperograms gave fundamental electrochemical parameters including the electroactive surface coverage (Gamma), the transfer coefficient (alpha), the standard rate constant (k(s)) and diffusion coefficient (D). Under optimized conditions in voltammetric method, the dynamic linear calibration curve for Cf was obtained in the concentration range of 40-1000 micromol/L with the detection limit of 6 micromol/L. The analytical performance of this sensor has been evaluated for detection of the analyte in urine and serum samples. Copyright (c) 2010 Elsevier B.V. All rights reserved.