Micronuclei as a biological endpoint for genotoxicity: A minireview.

The in vitro micronucleus assay has now been applied in many laboratories. This endpoint is useful in biomonitoring or ecotoxicology, as a sensitivity measure of human cells in cancer treatment and also to replace or supplement other in vitro genotoxicity assays. Learning more about the mechanisms of micronucleus formation allows conclusions about its biological significance. It has been demonstrated that disturbance of the mitotic apparatus (spindle, kinetochores) as well as impaired function of topoisomerase II can be involved in micronucleus formation. In addition, the roles of changes in DNA-conformation that are induced by alterations in the status of cytosine-methylation and of the cellular DNA repair capacity have been shown. The fate of micronucleus-containing cells is not known: the cells may theoretically be cytostatic and micronucleus-formation may therefore be a way of the organism to eliminate genetic damage or the cells may survive the loss of that chromosomal material and develop into transformed cells. Published data and ideas of selected areas within this field are reviewed.