Physiological adaptations of stressed fish to polluted environments: role of heat shock proteins.

Fish are subjected to a wide variety of environmental stressors. Stressors affect fish at all life stages and the stress-specific responses that occur at the biochemical and physiological levels affect the overall health and longevity of such animals. In this review, the organ-specific alterations in fish that inhabit polluted environments are addressed in detail. Fish,like other vertebrates, have evolved strategies to counteract stress-mediated effects. Among the key strategies that fish have developed is the induction of HSPs. The primary functions of HSPs are to promote the proper folding or refolding of proteins, to prevent potentially damaging interactions with proteins, and aiding in the disassembly of formations of protein aggregates. Stress, a state of unbalanced tissue oxidation, causes a general disturbance in the cellular antioxidant and redox balance and evokes HSP70 overexpression. Distinct families of HSPs have diverse physiological functions, and their induction, which is regulated at the transcriptional level, is mediated by the activation of heat shock factors. Interestingly, HSPs also interact with a wide variety of signaling molecules that modulate stress-mediated apoptotic effects. Hence, HSP induction is of major importance for maintenance of cell homeostasis. HSP-mediated adaptation processes are regarded as a fundamental protective mechanism that decreases cellular sensitivity to damaging events. Thus, the adaptive expression of HSPs is a protective response that helps combat stress-induced conformational damage to proteins. Additional research is needed to gain further information on the functional significance and role of individual HSPs and to enhance the understanding of the molecular mechanisms by which they act. In addition, field studies are needed to allow comprehensive evaluation of the potential use of HSPs as biomarkers for environmental monitoring. Furthermore, the expression of HSPs in fish fluctuates in response to seasonal variation. Because HSPs serves as a tool for assessing the stressed state of individuals and/or populations, the impact of seasonal influences on constitutive and inducible factors of these proteins should also be elucidated. Such research will lead to a fundamental improvement in the understanding of the functional role of HSPs in response to natural environmental changes and may allow correlation of the action of HSPs at the molecular level with the whole organismal stress response, which, so far, remains unexplained.