Li Xie1, Jia Wei, Xiaoping Qian, Gang Chen, Lixia Yu, Yitao Ding, Baorui Liu. 1. The Comprehensive Cancer Center, of Drum Tower Hospital, affiliated to the Medical School of Nanjing University and Clinical Cancer Institute of Nanjing University, Nanjing, PR China.
Abstract
UNLABELLED: The aim of this study was to explore predictive genes involved in docetaxel sensitivity of gastric cancer. MATERIALS AND METHODS: Microarray analysis was performed to explore various gene expression levels between parental and docetaxel-resistant cells. A panel of 11 genes selected according to microarray analysis were validated and tested further in 11 cancer cell lines, resulting in 4 genes, CXCR4, CDK6, USP15 and CDH1. Histoculture drug response assay (HDRA) was used to examine docetaxel sensitivity, while qRT-PCR was used to measure the mRNA levels of the genes in 25 surgically dissected gastric cancer specimens. RESULTS: Only CXCR4 mRNA levels in gastric cancer tissues were correlated with docetaxel sensitivity (R(2)=0.23, p=0.019) and significantly higher in resistant specimens (p=0.038). AMD3100, a CXCR4 antagonist, enhanced the docetaxel cytotoxicity in vitro. CONCLUSION: CXCR4 mRNA expression levels may be a potential predictive biomarker in gastric cancer.
UNLABELLED: The aim of this study was to explore predictive genes involved in docetaxel sensitivity of gastric cancer. MATERIALS AND METHODS: Microarray analysis was performed to explore various gene expression levels between parental and docetaxel-resistant cells. A panel of 11 genes selected according to microarray analysis were validated and tested further in 11 cancer cell lines, resulting in 4 genes, CXCR4, CDK6, USP15 and CDH1. Histoculture drug response assay (HDRA) was used to examine docetaxel sensitivity, while qRT-PCR was used to measure the mRNA levels of the genes in 25 surgically dissected gastric cancer specimens. RESULTS: Only CXCR4 mRNA levels in gastric cancer tissues were correlated with docetaxel sensitivity (R(2)=0.23, p=0.019) and significantly higher in resistant specimens (p=0.038). AMD3100, a CXCR4 antagonist, enhanced the docetaxelcytotoxicity in vitro. CONCLUSION:CXCR4 mRNA expression levels may be a potential predictive biomarker in gastric cancer.
Authors: Thomas Thomaidis; Annett Maderer; Salah-Eddin Al-Batran; Janis Kany; Claudia Pauligk; Kristina Steinmetz; Arno Schad; Ralf Hofheinz; Harald Schmalenberg; Nils Homann; Peter Robert Galle; Markus Moehler Journal: BMC Cancer Date: 2014-07-01 Impact factor: 4.430