Literature DB >> 20650858

[Genomic DNA extraction from hair sacs of pigs using modified phenol-chloroform method].

Ji-Ying Wang1, Ying Yu, Li-Xia Feng, Huai-Zhong Wang, Qin Zhang.   

Abstract

In referring to various methods for genomic DNA extraction from different tissues, we modified the classical phenol-chloroform procedure and reaction system for use in genomic DNA extraction from pig hair sacs. With the modified the phenol-chloroform method we successfully obtained high quality genomic DNA from pig hair sacs. Genomic DNA can be extracted from sacs of one to six pig hairs with satisfied quantity and quality for the need of PCR-based molecular ex-periment.

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Year:  2010        PMID: 20650858     DOI: 10.3724/sp.j.1005.2010.00752

Source DB:  PubMed          Journal:  Yi Chuan        ISSN: 0253-9772


  2 in total

1.  The Distribution Characteristics of a 19-bp Indel of the PLAG1 Gene in Chinese Cattle.

Authors:  Zihui Zhou; Bizhi Huang; Zhenyu Lai; Shipeng Li; Fei Wu; Kaixing Qu; Yutang Jia; Jiawen Hou; Jianyong Liu; Chuzhao Lei; Ruihua Dang
Journal:  Animals (Basel)       Date:  2019-12-04       Impact factor: 2.752

2.  Development and validation of a flexible DNA extraction (PAN) method for liquid biopsy of multiple sample types.

Authors:  Xiao-Xia Chen; Wei Chen; Yi-Long Liu; Can-Xiang Lin; Mengmeng Li; Wen-Jie Chen; Shang-Hang Xie; Dong-Feng Lin; Su-Mei Cao
Journal:  J Clin Lab Anal       Date:  2021-08-16       Impact factor: 2.352

  2 in total

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