OBJECTIVES: The aim of this study was to evaluate the cytotoxic effect of the monomers isobutyl methacrylate (IBMA) and 1,6-hexanediol dimethacrylate (1,6-HDMA), the plasticizer di-n-butyl phthalate (DBP), and the degradation by-products methacrylic acid (MA) and benzoic acid (BA) on L929 cells. Based on previous investigations on the release of these compounds from hard chairside reline resins, a range of concentrations (micromol/L) were selected for the cytotoxicity tests (IBMA, 5.49-1406.57; 1,6-HDMA, 1.22-39.32; DBP, 1.12-143.8; MA, 9.07-581; BA, 3.19-409). METHODS: Cytotoxic effects were assessed using MTT and (3)H-thymidine assays after the cells had been exposed to the test compounds at the given concentrations for 24 h. Cytotoxicity was rated based on cell viability relative to controls (cells exposed to medium without test substances). RESULTS: DNA synthesis activity was inhibited by all compounds. Mitochondrial dehydrogenase activity decreased in cells treated with monomers, plasticizer and MA by-product, whereas no cytotoxic effect was observed on contact with BA at the majority of concentrations tested. The ranges of suppression for (3)H-thymidine assay were: IBMA, 25-95%; 1,6-HDMA, 95-98%; DBP, 40-98%; MA, 97-99%; BA, 54-71%. For MTT assay, the ranges of suppression were: IBMA, 0-96%; 1,6-HDMA, 26-89%; DBP, 17-80%; MA, 52-66%; BA, 0-27%. The (3)H-thymidine assay was more sensitive than the MTT assay. SIGNIFICANCE: This study evaluated the cytotoxicity of a wide range of concentrations of monomers (IBMA and 1,6-HDMA), plasticizer (DBP) and degradation by-products (MA and BA), including those expected to be released from hard chairside reline resins. The differences observed in the cytotoxicity of these compounds, along with other properties, may assist the dental practitioners in the selection of reline materials with improved service life performance and low risk of adverse reactions in patients who wear relined dentures.
OBJECTIVES: The aim of this study was to evaluate the cytotoxic effect of the monomers isobutyl methacrylate (IBMA) and 1,6-hexanediol dimethacrylate (1,6-HDMA), the plasticizer di-n-butyl phthalate (DBP), and the degradation by-products methacrylic acid (MA) and benzoic acid (BA) on L929 cells. Based on previous investigations on the release of these compounds from hard chairside reline resins, a range of concentrations (micromol/L) were selected for the cytotoxicity tests (IBMA, 5.49-1406.57; 1,6-HDMA, 1.22-39.32; DBP, 1.12-143.8; MA, 9.07-581; BA, 3.19-409). METHODS:Cytotoxic effects were assessed using MTT and (3)H-thymidine assays after the cells had been exposed to the test compounds at the given concentrations for 24 h. Cytotoxicity was rated based on cell viability relative to controls (cells exposed to medium without test substances). RESULTS: DNA synthesis activity was inhibited by all compounds. Mitochondrial dehydrogenase activity decreased in cells treated with monomers, plasticizer and MA by-product, whereas no cytotoxic effect was observed on contact with BA at the majority of concentrations tested. The ranges of suppression for (3)H-thymidine assay were: IBMA, 25-95%; 1,6-HDMA, 95-98%; DBP, 40-98%; MA, 97-99%; BA, 54-71%. For MTT assay, the ranges of suppression were: IBMA, 0-96%; 1,6-HDMA, 26-89%; DBP, 17-80%; MA, 52-66%; BA, 0-27%. The (3)H-thymidine assay was more sensitive than the MTT assay. SIGNIFICANCE: This study evaluated the cytotoxicity of a wide range of concentrations of monomers (IBMA and 1,6-HDMA), plasticizer (DBP) and degradation by-products (MA and BA), including those expected to be released from hard chairside reline resins. The differences observed in the cytotoxicity of these compounds, along with other properties, may assist the dental practitioners in the selection of reline materials with improved service life performance and low risk of adverse reactions in patients who wear relined dentures.
Authors: Rasika P Illeperuma; Young J Park; Jin M Kim; Jung Y Bae; Zhong M Che; Hwa K Son; Mi R Han; Kwang M Kim; Jin Kim Journal: J Mater Sci Mater Med Date: 2011-11-10 Impact factor: 3.896
Authors: Carolina Al Chaves; Carlos E Vergani; Dominique Thomas; Anne Young; Carlos As Costa; Vehid M Salih; Ana L Machado Journal: J Tissue Eng Date: 2014-06-23 Impact factor: 7.813
Authors: Carolina de Andrade Lima Chaves; Carlos Alberto de Souza Costa; Carlos Eduardo Vergani; Pedro Paulo Chaves de Souza; Ana Lucia Machado Journal: Biomed Res Int Date: 2014-09-11 Impact factor: 3.411
Authors: Cristina B Neves; Luís P Lopes; Helena F Ferrão; Joana P Miranda; Matilde F Castro; Ana F Bettencourt Journal: Biomed Res Int Date: 2013-07-18 Impact factor: 3.411