Comparative study of normal and rough human skin hydration in vivo: evaluation with four different instruments.

Appropriate monitoring of skin hydration during clinical and/or experimental trials needs devices with acceptable reproducibility and sensitivity under conditions ranging from increased, and normal to low hydration. The aim of this study was to compare the variation of electrometric data generated by 4 different instruments (Skicon Hygrometer, 2 CM420 and a CM820 corneometer) in normal and experimentally damaged skin displaying surface roughness. Rough skin sites were observed during the healing process after repeated tape stripping of stratum corneum in humans (e.g. 10-14 days after insult). They displayed lower conductance and or capacitance levels as compared to normal skin sites of the same subjects. The Skicon hygrometer showed higher variability as compared to the corneometers and was less sensitive, in relative terms, in the rough skin sites. This device also showed a moderate zero drift and re-zeroing was repeatedly utilized during the experiment. When the corneometer data were plotted against the hygrometer data, the slope of the regression line generated by the CM420a was different from CM420b and from CM820; the two latter were not significantly different from each other. Hence, comparison of absolute data obtained under comparable conditions (in this case CM420a and CM420b) in a single laboratory should not be made without prior calibration. Standards for evaluating interinstrumental variation are currently unavailable. This aspect of the measurement of electrical properties of the skin has not been investigated in great detail and has often been neglected in the past. Our findings also indicate that a constant control over the performances of a particular device should further improve the reliability of the data.