Literature DB >> 2064765

Simplified procedure for transient transformation of plant protoplasts using polyethylene glycol treatment.

L A Lyznik1, J Y Peng, T K Hodges.   

Abstract

A modification of the polyethylene glycol-mediated transformation procedure which eliminates the manual polyethylene glycol dilution step is presented. A transformation mixture of protoplasts, DNA and polyethylene glycol was plated directly onto agarose blocks after incubation. The procedure was simple and fast, thereby suitable for screening the gene activity of large numbers of plasmid constructions. It has been tested for both maize and rice protoplasts.

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Year:  1991        PMID： 2064765

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  4 in total

1.  ACCUMULATION OF PHOTOSYSTEM ONE1, a member of a novel gene family, is required for accumulation of [4Fe-4S] cluster-containing chloroplast complexes and antenna proteins.

Authors:  Katrin Amann; Lina Lezhneva; Gerd Wanner; Reinhold G Herrmann; Jörg Meurer
Journal:  Plant Cell       Date:  2004-10-19       Impact factor: 11.277

2.  Recruitment of a ribosomal release factor for light- and stress-dependent regulation of petB transcript stability in Arabidopsis chloroplasts.

Authors:  Rhea Stoppel; Lina Lezhneva; Serena Schwenkert; Salar Torabi; Susanne Felder; Karin Meierhoff; Peter Westhoff; Jörg Meurer
Journal:  Plant Cell       Date:  2011-07-19       Impact factor: 11.277

3.  Homologous recombination between plasmid DNA molecules in maize protoplasts.

Authors:  L A Lyznik; J D McGee; P Y Tung; J L Bennetzen; T K Hodges
Journal:  Mol Gen Genet       Date:  1991-11

4.  A Novel Chloroplast Protein RNA Processing 8 Is Required for the Expression of Chloroplast Genes and Chloroplast Development in Arabidopsis thaliana.

Authors:  Mengmeng Kong; Yaozong Wu; Ziyuan Wang; Wantong Qu; Yixin Lan; Xin Chen; Yanyun Liu; Perveen Shahnaz; Zhongnan Yang; Qingbo Yu; Hualing Mi
Journal:  Front Plant Sci       Date:  2021-12-09       Impact factor: 5.753
  4 in total

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