BACKGROUND: Defects in lymphoproliferative responses to mitogens/antigens in women >45 years old with a persistent type-specific human papillomavirus (HPV) infection have been reported. METHODS: To determine whether these defects were associated with altered cytokine profiles, plasma and peripheral blood mononuclear cell (PBMC) culture supernatants from 50 cases (oversampled for their reduced lymphoproliferative ability) and 50 uninfected controls (oversampled for their robust lymphoproliferative ability) were examined for 24 cytokines using multiplexed bead-based immunoassays and ELISA. RESULTS: The following plasma cytokines were significantly increased in cases relative to controls (cases versus controls; median pg/mL): interleukin (IL)-6, 393.1 versus 14.5; IL-8, 1,128.5 versus 43.9; tumor necrosis factor-alpha (TNF-alpha), 164.1 versus 9.2; macrophage inflammatory protein-1alpha (MIP-1alpha), 1,368.9 versus 25.5; granulocyte macrophage colony-stimulating factor (GM-CSF), 13.8 versus 7.3; IL-1beta, 8.3 versus 1.6 (all P < 0.0001); and IL-1alpha, 218.2 versus 169.5 (P = 0.02). We focused our analysis on the cytokines IL-6, IL-8, TNF-alpha, and MIP-1alpha due to their high fold change (>10) and highly statistically significant difference between cases and controls. Length of persistence or type of infection (high risk and low risk) did not affect these differences. IL-6, TNF-alpha, and MIP-1alpha levels were also increased in unstimulated PBMC culture supernatants from cases compared with controls (P < 0.05), however, the cytokine levels from phytohemagglutinin-stimulated PBMC culture supernatants were significantly lower in the cases (P < 0.0001). CONCLUSIONS: Persistent HPV infection in older women with evidence of immune deficit is associated with an increase in systemic inflammatory cytokines. IMPACT: Future studies are needed to determine whether the inflammatory profile is age dependent and to examine the role that inflammatory cytokines play in HPV-induced progression from infection to cervical cancer. (c)2010 AACR.
BACKGROUND: Defects in lymphoproliferative responses to mitogens/antigens in women >45 years old with a persistent type-specific humanpapillomavirus (HPV) infection have been reported. METHODS: To determine whether these defects were associated with altered cytokine profiles, plasma and peripheral blood mononuclear cell (PBMC) culture supernatants from 50 cases (oversampled for their reduced lymphoproliferative ability) and 50 uninfected controls (oversampled for their robust lymphoproliferative ability) were examined for 24 cytokines using multiplexed bead-based immunoassays and ELISA. RESULTS: The following plasma cytokines were significantly increased in cases relative to controls (cases versus controls; median pg/mL): interleukin (IL)-6, 393.1 versus 14.5; IL-8, 1,128.5 versus 43.9; tumor necrosis factor-alpha (TNF-alpha), 164.1 versus 9.2; macrophage inflammatory protein-1alpha (MIP-1alpha), 1,368.9 versus 25.5; granulocyte macrophage colony-stimulating factor (GM-CSF), 13.8 versus 7.3; IL-1beta, 8.3 versus 1.6 (all P < 0.0001); and IL-1alpha, 218.2 versus 169.5 (P = 0.02). We focused our analysis on the cytokines IL-6, IL-8, TNF-alpha, and MIP-1alpha due to their high fold change (>10) and highly statistically significant difference between cases and controls. Length of persistence or type of infection (high risk and low risk) did not affect these differences. IL-6, TNF-alpha, and MIP-1alpha levels were also increased in unstimulated PBMC culture supernatants from cases compared with controls (P < 0.05), however, the cytokine levels from phytohemagglutinin-stimulated PBMC culture supernatants were significantly lower in the cases (P < 0.0001). CONCLUSIONS: Persistent HPV infection in older women with evidence of immune deficit is associated with an increase in systemic inflammatory cytokines. IMPACT: Future studies are needed to determine whether the inflammatory profile is age dependent and to examine the role that inflammatory cytokines play in HPV-induced progression from infection to cervical cancer. (c)2010 AACR.
Authors: Y S Cho; J W Kang; M Cho; C W Cho; S Lee; Y K Choe; Y Kim; I Choi; S N Park; S Kim; C A Dinarello; D Y Yoon Journal: FEBS Lett Date: 2001-07-20 Impact factor: 4.124
Authors: E Lazcano-Ponce; R Herrero; N Muñoz; A Cruz; K V Shah; P Alonso; P Hernández; J Salmerón; M Hernández Journal: Int J Cancer Date: 2001-02-01 Impact factor: 7.396
Authors: Silvia Franceschi; Rolando Herrero; Gary M Clifford; Peter J F Snijders; Annie Arslan; Pham Thi Hoang Anh; F Xavier Bosch; Catterina Ferreccio; Nguyen Trong Hieu; Eduardo Lazcano-Ponce; Elena Matos; Monica Molano; You-Lin Qiao; Raj Rajkumar; Guglielmo Ronco; Silvia de Sanjosé; Hai-Rim Shin; Sukhon Sukvirach; Jaiye O Thomas; Chris J L M Meijer; Nubia Muñoz Journal: Int J Cancer Date: 2006-12-01 Impact factor: 7.396
Authors: Alfonso J García-Piñeres; Allan Hildesheim; Rolando Herrero; Matthew Trivett; Marcus Williams; Ivannia Atmetlla; Margarita Ramírez; Maricela Villegas; Mark Schiffman; Ana Cecilia Rodríguez; Robert D Burk; Mariana Hildesheim; Enrique Freer; José Bonilla; Concepción Bratti; Jay A Berzofsky; Ligia A Pinto Journal: Cancer Res Date: 2006-11-15 Impact factor: 12.701
Authors: Sophia S Wang; Rosemary E Zuna; Nicolas Wentzensen; S Terence Dunn; Mark E Sherman; Michael A Gold; Mark Schiffman; Sholom Wacholder; Richard A Allen; Ingrid Block; Kim Downing; Jose Jeronimo; J Daniel Carreon; Mahboobeh Safaeian; David Brown; Joan L Walker Journal: Cancer Epidemiol Biomarkers Prev Date: 2009-01 Impact factor: 4.254
Authors: Morgan A Marks; Raphael P Viscidi; Kathryn Chang; Michelle Silver; Anne Burke; Roslyn Howard; Patti E Gravitt Journal: Cytokine Date: 2011-10-19 Impact factor: 3.861
Authors: Carole Fakhry; Fares Qeadan; Robert H Gilman; Pablo Yori; Margaret Kosek; Nicole Patterson; David W Eisele; Christine G Gourin; Chandala Chitguppi; Morgan Marks; Patti Gravitt Journal: Laryngoscope Date: 2017-11-24 Impact factor: 3.325
Authors: Anil K Chaturvedi; Troy J Kemp; Ruth M Pfeiffer; Angelique Biancotto; Marcus Williams; Stella Munuo; Mark P Purdue; Ann W Hsing; Ligia Pinto; J Philip McCoy; Allan Hildesheim Journal: Cancer Epidemiol Biomarkers Prev Date: 2011-06-29 Impact factor: 4.254
Authors: Rosalyn Baker; Joseph G Dauner; Ana Cecilia Rodriguez; Marcus C Williams; Troy J Kemp; Allan Hildesheim; Ligia A Pinto Journal: Cytokine Date: 2010-12-16 Impact factor: 3.861
Authors: Jennifer O Lam; Jay H Bream; Elizabeth A Sugar; Christian L Coles; Kathleen M Weber; Robert D Burk; Dorothy J Wiley; Ross D Cranston; Susheel Reddy; Joseph B Margolick; Howard D Strickler; Alicia Wentz; Lisa Jacobson; Yingshi Guo; Weihong Xiao; Maura L Gillison; Gypsyamber D'Souza Journal: Cytokine Date: 2016-04-08 Impact factor: 3.861
Authors: Sheetal Hardikar; Lisa G Johnson; Mari Malkki; Effie W Petersdorf; Denise A Galloway; Stephen M Schwartz; Margaret M Madeleine Journal: Gynecol Oncol Date: 2015-08-01 Impact factor: 5.482
Authors: Su-Hsun Liu; Anne F Rositch; Raphael P Viscidi; Michelle I Silver; Anne E Burke; Patti E Gravitt Journal: J Infect Dis Date: 2013-07-09 Impact factor: 5.226