| Literature DB >> 20647351 |
Martin Hülskamp, Birgit Schwab, Paul Grini, Heinz Schwarz.
Abstract
INTRODUCTION: Transmission electron microscopy (TEM) requires extensive preparation of plant tissues to achieve optimal results. Samples must be fixed to preserve ultrastructure, dehydrated to withstand the high vacuum of an electron microscope, and embedded in a suitable resin to permit very thin sections to be produced. This protocol provides details for fixation, dehydration with ethanol, and embedding in Spurr's, an epoxide resin with low viscosity that is well-suited for plant tissues.Entities:
Mesh：
Year: 2010 PMID： 20647351 DOI： 10.1101/pdb.prot4958
Source DB: PubMed Journal: Cold Spring Harb Protoc ISSN： 1559-6095