INTRODUCTION: The diagnosis and assessment of apical periodontitis by traditional periapical radiographs can be challenging and might yield false-negative results. The aim of this study was to determine whether interleukin-1beta (IL-1beta) and dentin sialoprotein (DSP) in gingival crevicular fluid (GCF) can be used as biological markers for apical periodontitis. METHODS: Forty healthy patients with teeth diagnosed with apical periodontitis of pulpal origin were included in the study. GCF samples were obtained from the diseased tooth and from a healthy contralateral control tooth. Total protein concentration in each sample was determined by using the Bio-Rad protein assay. Enzyme-linked immunosorbent assay was used to analyze the concentration of IL-1beta and DSP in the samples. RESULTS: Protein content of the GCF was statistically significantly higher in the disease group compared with the control group. The levels of IL-1beta and DSP were not statistically different between disease and control groups. CONCLUSIONS: Although this study was unable to demonstrate a significantly higher level of IL-1beta or DSP in the GCF of teeth with apical periodontitis, the observed presence of a significantly higher level of total protein in the GCF of diseased teeth suggests the possible role of total protein level as a marker for periapical disease. Copyright 2010 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
INTRODUCTION: The diagnosis and assessment of apical periodontitis by traditional periapical radiographs can be challenging and might yield false-negative results. The aim of this study was to determine whether interleukin-1beta (IL-1beta) and dentin sialoprotein (DSP) in gingival crevicular fluid (GCF) can be used as biological markers for apical periodontitis. METHODS: Forty healthy patients with teeth diagnosed with apical periodontitis of pulpal origin were included in the study. GCF samples were obtained from the diseased tooth and from a healthy contralateral control tooth. Total protein concentration in each sample was determined by using the Bio-Rad protein assay. Enzyme-linked immunosorbent assay was used to analyze the concentration of IL-1beta and DSP in the samples. RESULTS: Protein content of the GCF was statistically significantly higher in the disease group compared with the control group. The levels of IL-1beta and DSP were not statistically different between disease and control groups. CONCLUSIONS: Although this study was unable to demonstrate a significantly higher level of IL-1beta or DSP in the GCF of teeth with apical periodontitis, the observed presence of a significantly higher level of total protein in the GCF of diseased teeth suggests the possible role of total protein level as a marker for periapical disease. Copyright 2010 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
Authors: Rohit Agrawal; Monika Bhardwaj; Bonita Doley; Abhigyan Manas; Prabu Mahin Syed Ismail; Prajakta B Patil; Kishore K Singh Journal: J Family Med Prim Care Date: 2022-05-14
Authors: Jenna L Riis; Hedyeh Ahmadi; Olivia Silke; Steve W Granger; Crystal I Bryce; Douglas A Granger Journal: Front Immunol Date: 2020-08-28 Impact factor: 7.561