M G Gandolfi1, P Taddei, A Tinti, C Prati. 1. Laboratory of Biomaterials and Oral Pathology, Endodontic Clinical Section, Department of Odontostomatological Science, University of Bologna Department of Biochemistry, University of Bologna, Bologna, Italy. mgiovanna.gandolfi@unibo.it
Abstract
AIM: Apatite-forming ability, considered as an index of bioactivity (bond-to-bone ability), was tested on ProRoot MTA cement after immersion in phosphate-containing solution (DPBS). METHODOLOGY: Disk samples were prepared and immersed in DPBS for 10 min, 5 h, 1 and 7 days. The cement surface was studied by attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy, by micro-Raman spectroscopy and by environmental scanning electron microscope with energy dispersive X-ray (ESEM-EDX) analyses. The pH of the storage solution was also investigated. RESULTS: Spectroscopic analyses revealed calcium phosphate bands after 5-h immersion in DPBS. After 1 day, an even coating composed of apatite spherulites (0.1-0.8 micron diameter) was observed by ESEM/EDX. After 7 days, its thickness had increased. Apatite nucleation had already occurred after 5-h immersion. At this time, the presence of portlandite (i.e. Ca(OH)(2) , calcium hydroxide) on the cement surface was also observed; at longer times, this component was released into the medium, which underwent a remarkable pH increase. CONCLUSIONS: The study confirms the ability of ProRoot MTA to form a superficial layer of apatite within hours. The excellent bioactivity of ProRoot MTA might provide a significant clinical advantage over the traditional cements used for root-end or root-perforation repair.
AIM: Apatite-forming ability, considered as an index of bioactivity (bond-to-bone ability), was tested on ProRoot MTA cement after immersion in phosphate-containing solution (DPBS). METHODOLOGY: Disk samples were prepared and immersed in DPBS for 10 min, 5 h, 1 and 7 days. The cement surface was studied by attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy, by micro-Raman spectroscopy and by environmental scanning electron microscope with energy dispersive X-ray (ESEM-EDX) analyses. The pH of the storage solution was also investigated. RESULTS: Spectroscopic analyses revealed calcium phosphate bands after 5-h immersion in DPBS. After 1 day, an even coating composed of apatite spherulites (0.1-0.8 micron diameter) was observed by ESEM/EDX. After 7 days, its thickness had increased. Apatite nucleation had already occurred after 5-h immersion. At this time, the presence of portlandite (i.e. Ca(OH)(2) , calcium hydroxide) on the cement surface was also observed; at longer times, this component was released into the medium, which underwent a remarkable pH increase. CONCLUSIONS: The study confirms the ability of ProRoot MTA to form a superficial layer of apatite within hours. The excellent bioactivity of ProRoot MTA might provide a significant clinical advantage over the traditional cements used for root-end or root-perforation repair.
Authors: Li-Na Niu; Kai Jiao; Tian-da Wang; Wei Zhang; Josette Camilleri; Brian E Bergeron; Hai-Lan Feng; Jing Mao; Ji-Hua Chen; David H Pashley; Franklin R Tay Journal: J Dent Date: 2014-01-15 Impact factor: 4.379
Authors: M G Gandolfi; G Spagnuolo; F Siboni; A Procino; V Rivieccio; G A Pelliccioni; C Prati; S Rengo Journal: Clin Oral Investig Date: 2015-03-31 Impact factor: 3.573
Authors: Guo-Hua Li; Li-Na Niu; Wei Zhang; Mark Olsen; Gustavo De-Deus; Ashraf A Eid; Ji-Hua Chen; David H Pashley; Franklin R Tay Journal: Acta Biomater Date: 2013-12-07 Impact factor: 8.947