| Literature DB >> 20644934 |
Wei Liu1, Jianqun Lin, Xin Pang, Shuang Cui, Shuang Mi, Jianqiang Lin.
Abstract
A wide-host-range plasmid pTRUS containing a homologous rus gene under the control of Ptac promoter was constructed and transferred into Acidithiobacillus ferrooxidans ATCC19859 using conjugation gene transfer method to generate the engineered strain of A. ferrooxidans(pTRUS). The plasmid-based recombinant rus gene was successfully expressed. According to the results of real-time quantitative PCR assay, not only the transcription level of recombinant rus gene but also the transcription levels of the other genes of rus operon (cyc1, orf, coxB, coxA) were increased in A. ferrooxidans(pTRUS). The ferrous ion (Fe(2+)) oxidation activity was increased in A. ferrooxidans(pTRUS).Entities:
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Year: 2010 PMID： 20644934 DOI： 10.1007/s00284-010-9708-0
Source DB: PubMed Journal: Curr Microbiol ISSN： 0343-8651 Impact factor: 2.188