BACKGROUND: Microvesicles are involved in different pathological processes such as inflammation, coagulation and tumor progression. We intended to establish an immunoaffinity capture method for detecting microvesicles and bioactive effectors carried on them using a specific homemade monoclonal antibody AD-1. By this method we investigated the association of inflammation with platelet activation in patients with nonvalvular atrial fibrillation (NVAF). METHODS: A case-control study of 90 Chinese subjects selected in 3 groups: control, paroxysmal AF, and persistent AF. After capturing the microvesicles of serum using a specific monoclonal antibody AD-1, the amounts of LAP, IL-1β and P-selectin loaded on these microvesicles were quantified by either enzyme activity assay (LAP) or ELISA respectively. RESULTS: Compared with normal controls, the patients with persistent AF showed significantly increased serum levels of microvesicles (P<0.001), microvesicle-bound IL-1 β (P=0.019) and microvesicle-bound P-selectin (P=0.001). The latter two were significantly correlated with each other (r(2)=0.371, r=0.616, P<0.001). The microvesicle-bound IL-1β (β=0.570, P<0.001) and body weight (β=0.427, P=0.002) were as independent predictors of platelet activation. CONCLUSIONS: The method was easy and reproducible. Inflammation may be involved in the activation of platelets in NVAF. 2010 Elsevier B.V. All rights reserved.
BACKGROUND: Microvesicles are involved in different pathological processes such as inflammation, coagulation and tumor progression. We intended to establish an immunoaffinity capture method for detecting microvesicles and bioactive effectors carried on them using a specific homemade monoclonal antibody AD-1. By this method we investigated the association of inflammation with platelet activation in patients with nonvalvular atrial fibrillation (NVAF). METHODS: A case-control study of 90 Chinese subjects selected in 3 groups: control, paroxysmal AF, and persistent AF. After capturing the microvesicles of serum using a specific monoclonal antibody AD-1, the amounts of LAP, IL-1β and P-selectin loaded on these microvesicles were quantified by either enzyme activity assay (LAP) or ELISA respectively. RESULTS: Compared with normal controls, the patients with persistent AF showed significantly increased serum levels of microvesicles (P<0.001), microvesicle-bound IL-1 β (P=0.019) and microvesicle-bound P-selectin (P=0.001). The latter two were significantly correlated with each other (r(2)=0.371, r=0.616, P<0.001). The microvesicle-bound IL-1β (β=0.570, P<0.001) and body weight (β=0.427, P=0.002) were as independent predictors of platelet activation. CONCLUSIONS: The method was easy and reproducible. Inflammation may be involved in the activation of platelets in NVAF. 2010 Elsevier B.V. All rights reserved.
Authors: Chunxia Yao; Tina Veleva; Larry Scott; Shuyi Cao; Luge Li; Gong Chen; Prince Jeyabal; Xiaolu Pan; Katherina M Alsina; Issam Abu-Taha; Shokoufeh Ghezelbash; Corey L Reynolds; Ying H Shen; Scott A LeMaire; Wilhelm Schmitz; Frank U Müller; Ali El-Armouche; N Tony Eissa; Christine Beeton; Stanley Nattel; Xander H T Wehrens; Dobromir Dobrev; Na Li Journal: Circulation Date: 2018-11-13 Impact factor: 29.690
Authors: Larry Scott; Anke C Fender; Arnela Saljic; Luge Li; Xiaohui Chen; Xiaolei Wang; Dominik Linz; Jilu Lang; Mathias Hohl; Darragh Twomey; Thuy T Pham; Rodrigo Diaz-Lankenau; Mihail G Chelu; Markus Kamler; Mark L Entman; George E Taffet; Prashanthan Sanders; Dobromir Dobrev; Na Li Journal: Cardiovasc Res Date: 2021-06-16 Impact factor: 10.787