Hui Shen1, Jane C Goodall, J S Hill Gaston. 1. First Affiliated Hospital of China Medical University - Rheumatology, Shen Yang, China. shenhuicam@sohu.com
Abstract
OBJECTIVE: To analyze the frequency and phenotype of peripheral blood mononuclear cells (PBMC) and synovial fluid mononuclear cell (SFMC) T helper (Th)17 cells in reactive arthritis (ReA). METHODS: T cell surface phenotype and cytokine production were measured following stimulation, using 8-color flow cytometry. RESULTS: The percentages of interleukin 17 (IL-17)-positive CD4+ T cells were increased in SFMC of patients with ReA compared with PBMC. All IL-17+ cells were CD4+CD45RO+; in SFMC most expressed CCR6, but only 50% expressed CCR4. IL-17+ cells sometimes coexpressed IL-22 and/or interferon-γ, but not IL-10. CONCLUSION: These data support the hypothesis that Th17 cells are involved in ReA pathogenesis.
OBJECTIVE: To analyze the frequency and phenotype of peripheral blood mononuclear cells (PBMC) and synovial fluid mononuclear cell (SFMC) T helper (Th)17 cells in reactive arthritis (ReA). METHODS: T cell surface phenotype and cytokine production were measured following stimulation, using 8-color flow cytometry. RESULTS: The percentages of interleukin 17 (IL-17)-positive CD4+ T cells were increased in SFMC of patients with ReA compared with PBMC. All IL-17+ cells were CD4+CD45RO+; in SFMC most expressed CCR6, but only 50% expressed CCR4. IL-17+ cells sometimes coexpressed IL-22 and/or interferon-γ, but not IL-10. CONCLUSION: These data support the hypothesis that Th17 cells are involved in ReA pathogenesis.
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